Knockdown of Hotair suppresses proliferation and cell cycle progression in hepatocellular carcinoma cell by downregulating CCND1 expression

  • Authors:
    • Jia‑Jia Zhou
    • Di Cheng
    • Xiao‑Yu He
    • Zhe Meng
    • Wen‑Zhu Li
    • Ru‑Fu Chen
  • View Affiliations

  • Published online on: August 3, 2017     https://doi.org/10.3892/mmr.2017.7162
  • Pages: 4980-4986
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Abstract

The long noncoding RNA, homeobox transcript antisense RNA (Hotair), has been demonstrated to have an important role in regulating various biological processes in various cancers, including hepatocellular carcinoma (HCC). However, the importance of Hotair in HCC proliferation and cell cycle progression remains to be elucidated. In the present study, knockdown of HOTAIR expression by RNA interference inhibited cell proliferation and induced G0/G1 cell cycle arrest in Huh7 hepatocellular carcinoma cells. In addition, the expression levels of CCND1 mRNA and its cyclin D1 protein product were reduced in Huh7 cells following knockdown of HOTAIR. Knockdown of HOTAIR reduced the expression of phosphorylated signal transducer and activator of transcription 3 (STAT3) and HOTAIR knockdown combined with STAT3 inhibition led to an additional decrease in cyclin D1 expression. The present study suggested that Hotair may have a critical role in the proliferation of HCC by regulating cell cycle, STAT3 activity and cyclin D1 expression. Therefore, Hotair may be a novel potential therapeutic target for HCC treatment.

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October-2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhou JJ, Cheng D, He XY, Meng Z, Li WZ and Chen RF: Knockdown of Hotair suppresses proliferation and cell cycle progression in hepatocellular carcinoma cell by downregulating CCND1 expression. Mol Med Rep 16: 4980-4986, 2017
APA
Zhou, J., Cheng, D., He, X., Meng, Z., Li, W., & Chen, R. (2017). Knockdown of Hotair suppresses proliferation and cell cycle progression in hepatocellular carcinoma cell by downregulating CCND1 expression. Molecular Medicine Reports, 16, 4980-4986. https://doi.org/10.3892/mmr.2017.7162
MLA
Zhou, J., Cheng, D., He, X., Meng, Z., Li, W., Chen, R."Knockdown of Hotair suppresses proliferation and cell cycle progression in hepatocellular carcinoma cell by downregulating CCND1 expression". Molecular Medicine Reports 16.4 (2017): 4980-4986.
Chicago
Zhou, J., Cheng, D., He, X., Meng, Z., Li, W., Chen, R."Knockdown of Hotair suppresses proliferation and cell cycle progression in hepatocellular carcinoma cell by downregulating CCND1 expression". Molecular Medicine Reports 16, no. 4 (2017): 4980-4986. https://doi.org/10.3892/mmr.2017.7162