Open Access

Comparative proteomic analysis of rats subjected to water immersion and restraint stress as an insight into gastric ulcers

  • Authors:
    • Zheng‑Rong Zhou
    • Pan Huang
    • Guang‑Hao Song
    • Zhuang Zhang
    • Ke An
    • Han‑Wen Lu
    • Xiao‑Li Ju
    • Wei Ding
  • View Affiliations

  • Published online on: August 14, 2017     https://doi.org/10.3892/mmr.2017.7241
  • Pages: 5425-5433
  • Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In the present study, comparative proteomic analysis was performed in rats subjected to water immersion‑restraint stress (WRS). A total of 26 proteins were differentially expressed and identified using matrix‑assisted laser desorption/ionization time of flight mass spectrometry. Among the 26 differentially expressed protein spots identified, 13 proteins were significantly upregulated under WRS, including pyruvate kinase and calreticulin, which may be closely associated with energy metabolism. In addition, 12 proteins were downregulated under WRS, including hemoglobin subunit β‑2 and keratin type II cytoskeletal 8, which may be important in protein metabolism and cell death. Gene Ontology analysis revealed the cellular distribution, molecular function and biological processes of the identified proteins. The mRNA levels of certain differentially expressed proteins were analyzed using fluorescence quantitative polymerase chain reaction analysis. The results of the present study aimed to offer insights into proteins, which are differentially expressed in gastric ulcers in stress, and provide theoretical evidence of a radical cure for gastric ulcers in humans.

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October-2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhou ZR, Huang P, Song GH, Zhang Z, An K, Lu HW, Ju XL and Ding W: Comparative proteomic analysis of rats subjected to water immersion and restraint stress as an insight into gastric ulcers. Mol Med Rep 16: 5425-5433, 2017
APA
Zhou, Z., Huang, P., Song, G., Zhang, Z., An, K., Lu, H. ... Ding, W. (2017). Comparative proteomic analysis of rats subjected to water immersion and restraint stress as an insight into gastric ulcers. Molecular Medicine Reports, 16, 5425-5433. https://doi.org/10.3892/mmr.2017.7241
MLA
Zhou, Z., Huang, P., Song, G., Zhang, Z., An, K., Lu, H., Ju, X., Ding, W."Comparative proteomic analysis of rats subjected to water immersion and restraint stress as an insight into gastric ulcers". Molecular Medicine Reports 16.4 (2017): 5425-5433.
Chicago
Zhou, Z., Huang, P., Song, G., Zhang, Z., An, K., Lu, H., Ju, X., Ding, W."Comparative proteomic analysis of rats subjected to water immersion and restraint stress as an insight into gastric ulcers". Molecular Medicine Reports 16, no. 4 (2017): 5425-5433. https://doi.org/10.3892/mmr.2017.7241