Ca2+/Mg2+ homeostasis‑related TRPM7 channel mediates chondrocyte hypertrophy via regulation of the PI3K‑Akt signaling pathway

  • Authors:
    • Daigang Lu
    • Jining Qu
    • Liang Sun
    • Qiang Li
    • Hua Ling
    • Na Yang
    • Teng Ma
    • Qian Wang
    • Ming Li
    • Kun Zhang
    • Zhong Li
  • View Affiliations

  • Published online on: August 21, 2017     https://doi.org/10.3892/mmr.2017.7300
  • Pages: 5699-5705
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Abstract

Chondrocytes are specialized cells that form cartilage tissue, and are able to respond to their osmotic environment and exercise important roles in endochondral ossification via undergoing proliferation, hypertrophy and apoptosis. The transient receptor melastatin potential 7 (TRPM7) cation channel can modulate the intracellular and extracellular levels of Ca2+ and Mg2+, and therefore the cellular osmotic environment. However, the molecular pathways involved in TRPM7‑mediated signal transduction have yet to be elucidated. In the present study, the expression and functionality of TRPM7 were investigated during chondrocyte proliferation and hypertrophy. The ATDC5 mouse cell line was employed and cellular viability was evaluated using the MTT assay, whereas hypertrophy was monitored via evaluating the expression of chondrogenic marker genes and the activity of alkaline phosphatase (ALP). Gene expression of TRPM7 appeared slightly upregulated during the proliferative stages of chondrocyte development, and significantly upregulated during the hypertrophic stages, suggesting the importance of Ca2+/Mg2+ homeostasis for chondrocyte growth. Low extracellular Ca2+/Mg2+ levels significantly reduced the expression of type X collagen, Indian hedgehog homolog (Ihh) and matrix metalloproteinase (MMP)‑13 genes, as well as ALP activity; however, cell viability remained unaffected. Conversely, the gene expression levels of TRPM7 appeared upregulated in ATDC5 cells under low extracellular Ca2+ or Mg2+ conditions. Silencing TRPM7 expression during the chondrocyte differentiation period also reduced type X collagen, Ihh and MMP‑13 gene expression, and ALP activity. Furthermore, the phosphatidylinositol‑4,5‑bisphosphate 3‑kinase (PI3K)‑Akt signaling pathway was activated following TRPM7 overexpression, and inhibited following TRPM7 silencing. Notably, the actions of TRPM7 on chondrocyte hypertrophy were abolished through the inhibition of PI3K‑Akt signaling. The present results suggested that TRPM7 may be involved in Ca2+/Mg2+ homeostasis during chondrocyte hypertrophy, and contribute to endochondral ossification via interacting with the PI3K‑Akt signaling pathway.

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October-2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Lu D, Qu J, Sun L, Li Q, Ling H, Yang N, Ma T, Wang Q, Li M, Zhang K, Zhang K, et al: Ca2+/Mg2+ homeostasis‑related TRPM7 channel mediates chondrocyte hypertrophy via regulation of the PI3K‑Akt signaling pathway. Mol Med Rep 16: 5699-5705, 2017
APA
Lu, D., Qu, J., Sun, L., Li, Q., Ling, H., Yang, N. ... Li, Z. (2017). Ca2+/Mg2+ homeostasis‑related TRPM7 channel mediates chondrocyte hypertrophy via regulation of the PI3K‑Akt signaling pathway. Molecular Medicine Reports, 16, 5699-5705. https://doi.org/10.3892/mmr.2017.7300
MLA
Lu, D., Qu, J., Sun, L., Li, Q., Ling, H., Yang, N., Ma, T., Wang, Q., Li, M., Zhang, K., Li, Z."Ca2+/Mg2+ homeostasis‑related TRPM7 channel mediates chondrocyte hypertrophy via regulation of the PI3K‑Akt signaling pathway". Molecular Medicine Reports 16.4 (2017): 5699-5705.
Chicago
Lu, D., Qu, J., Sun, L., Li, Q., Ling, H., Yang, N., Ma, T., Wang, Q., Li, M., Zhang, K., Li, Z."Ca2+/Mg2+ homeostasis‑related TRPM7 channel mediates chondrocyte hypertrophy via regulation of the PI3K‑Akt signaling pathway". Molecular Medicine Reports 16, no. 4 (2017): 5699-5705. https://doi.org/10.3892/mmr.2017.7300