Open Access

MicroRNA‑141 inhibits epithelial‑mesenchymal transition, and ovarian cancer cell migration and invasion

  • Authors:
    • Qinghua Ye
    • Lei Lei
    • Lingyun Shao
    • Jing Shi
    • Jun Jia
    • Xiaowen Tong
  • View Affiliations

  • Published online on: September 13, 2017     https://doi.org/10.3892/mmr.2017.7482
  • Pages: 6743-6749
  • Copyright: © Ye et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The effects of microRNA‑141 (miR‑141) on epithelial‑mesenchymal transition (EMT), and ovarian cancer cell migration and invasion were investigated. SKOV3 cells were transfected with the miR‑141 mimic (mimic group), inhibitor (inhibitor group) and nonspecific sequences (NC group), and left untransfected group (blank group). The reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was used to detect the expression of miR‑141 in SKOV3 cell lines. Then, mRNA levels and protein expression of EMT markers were determined by RT‑qPCR and western blotting, respectively. Cell proliferation was assessed using an MTT assay, followed by analysis of cell invasion and migration. SPSS software was used for statistical analysis. The results demonstrated that miR‑141 expression in the mimic group was increased compared with the NC or blank group. Compared with the NC or blank group, upregulation of epithelial‑cadherin (E‑cadherin) and integrin‑β, and downregulation of zinc finger E‑box‑binding homeobox (ZEB) was observed in the mimic group. The rate of cell proliferation decreased in the mimic group and increased in the inhibitor group when compared with the NC group (P<0.05). The number of invasive cells significantly increased in the inhibitor group and decreased in the mimic group when compared with the NC group (P<0.01). Compared with the NC group, the migratory rate was decreased in the mimic group, and increased in the inhibitor group at 24 and 48 h (all P<0.01). In conclusion, overexpression of miR‑141 caused upregulation of E‑cadherin, inhibited cell proliferation and EMT, and decreased cell invasion and migration in the SKOV3 cell line.
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November-2017
Volume 16 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Ye Q, Lei L, Shao L, Shi J, Jia J and Tong X: MicroRNA‑141 inhibits epithelial‑mesenchymal transition, and ovarian cancer cell migration and invasion. Mol Med Rep 16: 6743-6749, 2017
APA
Ye, Q., Lei, L., Shao, L., Shi, J., Jia, J., & Tong, X. (2017). MicroRNA‑141 inhibits epithelial‑mesenchymal transition, and ovarian cancer cell migration and invasion. Molecular Medicine Reports, 16, 6743-6749. https://doi.org/10.3892/mmr.2017.7482
MLA
Ye, Q., Lei, L., Shao, L., Shi, J., Jia, J., Tong, X."MicroRNA‑141 inhibits epithelial‑mesenchymal transition, and ovarian cancer cell migration and invasion". Molecular Medicine Reports 16.5 (2017): 6743-6749.
Chicago
Ye, Q., Lei, L., Shao, L., Shi, J., Jia, J., Tong, X."MicroRNA‑141 inhibits epithelial‑mesenchymal transition, and ovarian cancer cell migration and invasion". Molecular Medicine Reports 16, no. 5 (2017): 6743-6749. https://doi.org/10.3892/mmr.2017.7482