Open Access

Promoting potential of adipose derived stem cells on peripheral nerve regeneration

  • Authors:
    • Jiayan Guo
    • Shu Guo
    • Yuxin Wang
    • Yanqiu Yu
  • View Affiliations

  • Published online on: September 21, 2017     https://doi.org/10.3892/mmr.2017.7570
  • Pages: 7297-7304
  • Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The ultimate goal of treating peripheral nerve defects is reconstructing continuity of the nerve stumps to regain nerve conduction and functional recovery. Clinically, autologous nerve grafts and Schwann cell (SC) therapy have limitations, such as the need for secondary surgery, sacrifice of another nerve and donor site complication. Adipose derived stem cells (ADSCs) may promise to be ideal alternative cells of SCs. To explore the potential of ADSCs promoting peripheral nerve regeneration, the present study investigated the influences of ADSCs on proliferation and neurotrophic function of SCs using co‑culture model in vitro. Western blot analysis, immunocytochemistry, a cell viability assay, reverse transcription‑polymerase chain reaction (RT‑PCR) and ELISA were applied for examining the interaction of ADSCs and SCs in a co‑culture model in vitro. Western blot analysis and immunocytochemistry demonstrated that protein expression levels of glial filament acidic protein (GFAP) and S100 in ADSCs co‑cultured with SCs for 14 days were significantly higher compared with cells cultured alone. Cell viability assay indicated that the cell viability of SCs co‑cultured with ADSCs for 3, 4, 5, 6 and 7 days was significantly higher than those cultured alone. RT‑PCR showed that expression levels of neurotrophic factors [nerve growth factor (NGF) and glial cell line‑derived neurotrophic factor (GDNF)] and extracellular matrix components [fibronectin (FN) and laminin (LN)] in SCs co‑cultured with ADSCs for 14 days were significantly higher than those in SCs cultured alone. NGF, GDNF, FN and LN in the supernatants of co‑culture system were significantly higher than cells cultured alone, as ELISA revealed. The results of this study suggested that the transplantation of ADSCs may have a promoting potential to the peripheral nerve regeneration as undifferentiated state.
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November-2017
Volume 16 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Guo J, Guo S, Wang Y and Yu Y: Promoting potential of adipose derived stem cells on peripheral nerve regeneration. Mol Med Rep 16: 7297-7304, 2017
APA
Guo, J., Guo, S., Wang, Y., & Yu, Y. (2017). Promoting potential of adipose derived stem cells on peripheral nerve regeneration. Molecular Medicine Reports, 16, 7297-7304. https://doi.org/10.3892/mmr.2017.7570
MLA
Guo, J., Guo, S., Wang, Y., Yu, Y."Promoting potential of adipose derived stem cells on peripheral nerve regeneration". Molecular Medicine Reports 16.5 (2017): 7297-7304.
Chicago
Guo, J., Guo, S., Wang, Y., Yu, Y."Promoting potential of adipose derived stem cells on peripheral nerve regeneration". Molecular Medicine Reports 16, no. 5 (2017): 7297-7304. https://doi.org/10.3892/mmr.2017.7570