Open Access

miR‑647 and miR‑1914 promote cancer progression equivalently by downregulating nuclear factor IX in colorectal cancer

Corrigendum in: /10.3892/mmr.2022.12713

  • Authors:
    • Shaoqing Liu
    • Dingding Qu
    • Weiping Li
    • Chenxiang He
    • Shisen Li
    • Guosheng Wu
    • Qingchuan Zhao
    • Liangliang Shen
    • Jian Zhang
    • Jianyong Zheng
  • View Affiliations

  • Published online on: September 29, 2017     https://doi.org/10.3892/mmr.2017.7675
  • Pages: 8189-8199
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRNAs/miRs) have been investigated as diagnostic and prognostic biomarkers for cancer; however, the significance of miRNAs in colorectal cancer (CRC) remains to be elucidated. The aim of the present study was to determine the genetic profiles of CRC tissue, and screen for miRNAs implicated in CRC cell proliferation and migration. RNA sequencing of 10 paired specimens was performed to for screen genes that were upregulated or downregulated in CRC. miRNA expression in CRC specimens and cell lines was confirmed using qPCR analysis. The significance of indicated miRNAs in CRC cell proliferation and migration was evaluated using MTT and scratch wound‑healing assays. Online computational prediction, isobaric tags for relative and absolute quantification analysis and a luciferase reporter assay were applied to determine candidate targeted genes for the miRNAs. RNA‑seq data revealed miR‑1914 as the most prominent miRNA in CRC specimens. qPCR analysis also suggested that the expression of miR‑1914, as well as its counterpart miR‑647 were elevated in CRC specimens and cell lines. Suppression of miR‑647/1914 using small interfering RNAs inhibited CRC SW480 and SW620 cell proliferation, and migration. Nuclear factor I/X (NFIX) was demonstrated to be a candidate for miR‑647/1914 and mediated the oncogenic activity of miR‑647/1914. In all, miR‑647 and miR‑1914 were demonstrated to promote the proliferation and migration of CRC cells by directly targeting NFIX. Therapeutic delivery of siRNAs targeting miR‑647/1914 and overexpression of NFIX may be feasible approaches for CRC treatment.
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December-2017
Volume 16 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Liu S, Qu D, Li W, He C, Li S, Wu G, Zhao Q, Shen L, Zhang J, Zheng J, Zheng J, et al: miR‑647 and miR‑1914 promote cancer progression equivalently by downregulating nuclear factor IX in colorectal cancer Corrigendum in /10.3892/mmr.2022.12713. Mol Med Rep 16: 8189-8199, 2017
APA
Liu, S., Qu, D., Li, W., He, C., Li, S., Wu, G. ... Zheng, J. (2017). miR‑647 and miR‑1914 promote cancer progression equivalently by downregulating nuclear factor IX in colorectal cancer Corrigendum in /10.3892/mmr.2022.12713. Molecular Medicine Reports, 16, 8189-8199. https://doi.org/10.3892/mmr.2017.7675
MLA
Liu, S., Qu, D., Li, W., He, C., Li, S., Wu, G., Zhao, Q., Shen, L., Zhang, J., Zheng, J."miR‑647 and miR‑1914 promote cancer progression equivalently by downregulating nuclear factor IX in colorectal cancer Corrigendum in /10.3892/mmr.2022.12713". Molecular Medicine Reports 16.6 (2017): 8189-8199.
Chicago
Liu, S., Qu, D., Li, W., He, C., Li, S., Wu, G., Zhao, Q., Shen, L., Zhang, J., Zheng, J."miR‑647 and miR‑1914 promote cancer progression equivalently by downregulating nuclear factor IX in colorectal cancer Corrigendum in /10.3892/mmr.2022.12713". Molecular Medicine Reports 16, no. 6 (2017): 8189-8199. https://doi.org/10.3892/mmr.2017.7675