Open Access

ID1 contributes to cell growth invasion and migration in salivary adenoid cystic carcinoma

  • Authors:
    • Xiao‑Meng Hu
    • Ting Lin
    • Xiao‑Yu Huang
    • Rui‑Huan Gan
    • Yong Zhao
    • Yan Feng
    • Lin‑Can Ding
    • Bo‑Hua Su
    • Da‑Li Zheng
    • You‑Guang Lu
  • View Affiliations

  • Published online on: October 9, 2017     https://doi.org/10.3892/mmr.2017.7744
  • Pages:8907-8915
  • Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previous studies have reported that inhibitor of DNA binding 1 (ID1) exerts an oncogenic role in a number of tumors. In the present study, the role of ID1 in the growth, invasion and migration of salivary adenoid cystic carcinoma (SACC) cells was investigated. ID1 expression in clinical SACC samples was compared with that in normal salivary tissues using immunohistochemical staining, and the correlation between ID1 expression and clinical pathological characteristics was then determined. Subsequently, ID1 was overexpressed or silenced to investigate the effects of ID1 expression on SACC cell proliferation, invasion and migration. In addition, the gene expression levels of known ID1 target genes, including S100A9, CDKN2A and matrix metalloproteinase 1 (MMP1) was measured using reverse transcription‑quantitative polymerase chain reaction to elucidate the potential mechanisms of ID1 in SACC. The results of the present study indicated that the protein expression levels of ID1 were significantly increased in the SACC tissues compared with that in the normal salivary tissues (P<0.001), and a positive correlation between ID1 expression and tumor stage (P=0.001), tumor invasion (P=0.002) and metastasis (P=0.019) in SACC was observed. Knockdown of ID1 in SACC cells significantly inhibited cell growth, invasion and migration (all P<0.01), whereas overexpression of ID1 promoted cell proliferation, invasion and migration (all P<0.01). The gene expression level of MMP1 was significantly reduced following ID1 knockdown in SACC‑83 cells when compared with negative controls (P<0.05), whereas S100A9 and CDKN2A expression levels were significantly upregulated (both P<0.05). The results suggest that ID1 may regulate the growth, invasion and migration of SACC cells, and that MMP1, S100A9 and CDKN2A may serve as target genes of ID1 and mediate the effects of ID1 in SACC cells. Therefore, ID1 may present a potential target gene for the treatment of patients with SACC to inhibit cancer cell growth and metastasis.

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December 2017
Volume 16 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

2016 Impact Factor: 1.692
Ranked #19/128 Medicine Research and Experimental
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APA
Hu, X., Lin, T., Huang, X., Gan, R., Zhao, Y., Feng, Y. ... Lu, Y. (2017). ID1 contributes to cell growth invasion and migration in salivary adenoid cystic carcinoma. Molecular Medicine Reports, 16, 8907-8915. https://doi.org/10.3892/mmr.2017.7744
MLA
Hu, X., Lin, T., Huang, X., Gan, R., Zhao, Y., Feng, Y., Ding, L., Su, B., Zheng, D., Lu, Y."ID1 contributes to cell growth invasion and migration in salivary adenoid cystic carcinoma". Molecular Medicine Reports 16.6 (2017): 8907-8915.
Chicago
Hu, X., Lin, T., Huang, X., Gan, R., Zhao, Y., Feng, Y., Ding, L., Su, B., Zheng, D., Lu, Y."ID1 contributes to cell growth invasion and migration in salivary adenoid cystic carcinoma". Molecular Medicine Reports 16, no. 6 (2017): 8907-8915. https://doi.org/10.3892/mmr.2017.7744