MicroRNA‑27a promotes tumorigenesis via targeting AKT in triple negative breast cancer

  • Authors:
    • Jing Wu
    • Zhihui Sun
    • Huijie Sun
    • Yanhua Li
  • View Affiliations

  • Published online on: October 26, 2017     https://doi.org/10.3892/mmr.2017.7886
  • Pages: 562-570
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Altered microRNA (miRNA/miR) expression regulates tumor development and progression in triple‑negative breast cancer (TNBC). The present study examined the effect of miR‑27a on proliferation, migration and invasion of TNBC cells in vitro and in vivo. An MTT assay was performed to examine the proliferation of MDA‑MB‑231 and MDA‑MB‑468 breast cancer cells with either overexpression of miR‑27a or downregulation of miR‑27a, in the presence or absence of radiation. The migratory and invasive abilities of MDA‑MB‑231 and MDA‑MB‑468 breast cancer cells were assessed by Transwell migration and Matrigel invasion assays. The protein expression levels were examined by western blotting. The caspase‑Glo3/7 assay was performed to examine the effect of miR‑27a on radiation‑induced apoptosis in MDA‑MB‑231 and MDA‑MB‑468 breast cancer cells. A luciferase assay was performed to evaluate the effect of miR‑27a on phosphatase and tensin homolog (PTEN) and B cell lymphoma (Bcl)‑2 associated X, apoptosis regulator (BAX) expression. Immunodeficient nude mice were used to examine tumor growth following injection of MDA‑MB‑231 breast cancer cells. miR‑27a promoted proliferation in vitro and in vivo, and enhanced migration and invasion in TNBC cells. miR‑27a improved the survival of TNBC cells following irradiation. miR‑27a inhibited radiation‑induced apoptosis in TNBC cells by regulation of caspase 3/7 and Bcl‑2 expression. Furthermore, the expression levels of PTEN and phosphorylated protein kinase B in MDA‑MB‑231 and MDA‑MB‑468 cells was altered following overexpression of miR‑27a. The luciferase assay demonstrated that miR‑27a regulated PTEN and BAX expression by binding to 3'‑untranslated regions. Overall, miR‑27a exhibits an essential role in tumor development and progression in TNBC and may be used as a potential biomarker to predict radiotherapy response and prognosis for the disease.
View Figures
View References

Related Articles

Journal Cover

January-2018
Volume 17 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wu J, Sun Z, Sun H and Li Y: MicroRNA‑27a promotes tumorigenesis via targeting AKT in triple negative breast cancer. Mol Med Rep 17: 562-570, 2018
APA
Wu, J., Sun, Z., Sun, H., & Li, Y. (2018). MicroRNA‑27a promotes tumorigenesis via targeting AKT in triple negative breast cancer. Molecular Medicine Reports, 17, 562-570. https://doi.org/10.3892/mmr.2017.7886
MLA
Wu, J., Sun, Z., Sun, H., Li, Y."MicroRNA‑27a promotes tumorigenesis via targeting AKT in triple negative breast cancer". Molecular Medicine Reports 17.1 (2018): 562-570.
Chicago
Wu, J., Sun, Z., Sun, H., Li, Y."MicroRNA‑27a promotes tumorigenesis via targeting AKT in triple negative breast cancer". Molecular Medicine Reports 17, no. 1 (2018): 562-570. https://doi.org/10.3892/mmr.2017.7886