Open Access

Effects of Shield1 on the viral replication of varicella‑zoster virus containing FKBP‑tagged ORF4 and 48

  • Authors:
    • Shuying Li
    • Zhanjun Liu
    • Ji Li
    • Aihua Liu
    • Lihua Zhu
    • Kui Yu
    • Ke Zhang
  • View Affiliations

  • Published online on: November 6, 2017     https://doi.org/10.3892/mmr.2017.7986
  • Pages: 763-770
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The present study aimed to explore the effects of a stabilizing ligand, Shield‑1, on the replication of recombinant varicella‑zoster virus (VZV) containing FK506 binding protein (FKPB) tags in essential open reading frames (ORF) 4 and 48. A specific galactokinase (galK) selection method was conducted, following the addition of galK labels to VZV ORF4 and 48, using a SW102 VZV bacterial artificial chromosome (BAC) system. Subsequently, recombinant VZV containing FKPB tags in ORF4 and 48 was constructed by counterselection and homologous recombination. Recombinant viral plasmids containing FKPB‑tagged VZV ORF4 and 48 were extracted and transfected into human acute retinal pigment epithelial ARPE‑19 cells. The results demonstrated that the FKPB‑tagged viral protein was rapidly degraded by proteases in recombinant virus‑infected ARPE‑19 cells. In addition, the recombinant VZVORF4‑FKBP‑ORF48‑FKBP virus could not grow if a synthetic ligand of FKBP, Shield1, was not added to the ARPE‑19 cell culture medium; however, the degradation of FKPB‑tagged viral protein was prevented if Shield1 was added to the ARPE‑19 cell culture medium, thereby allowing viral replication in ARPE‑19 cells. These results indicated that Shield1 may regulate replication of recombinant VZVORF4‑FKBP‑ORF48‑FKBP following transfection into human epithelial cells.
View Figures
View References

Related Articles

Journal Cover

January-2018
Volume 17 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Li S, Liu Z, Li J, Liu A, Zhu L, Yu K and Zhang K: Effects of Shield1 on the viral replication of varicella‑zoster virus containing FKBP‑tagged ORF4 and 48. Mol Med Rep 17: 763-770, 2018
APA
Li, S., Liu, Z., Li, J., Liu, A., Zhu, L., Yu, K., & Zhang, K. (2018). Effects of Shield1 on the viral replication of varicella‑zoster virus containing FKBP‑tagged ORF4 and 48. Molecular Medicine Reports, 17, 763-770. https://doi.org/10.3892/mmr.2017.7986
MLA
Li, S., Liu, Z., Li, J., Liu, A., Zhu, L., Yu, K., Zhang, K."Effects of Shield1 on the viral replication of varicella‑zoster virus containing FKBP‑tagged ORF4 and 48". Molecular Medicine Reports 17.1 (2018): 763-770.
Chicago
Li, S., Liu, Z., Li, J., Liu, A., Zhu, L., Yu, K., Zhang, K."Effects of Shield1 on the viral replication of varicella‑zoster virus containing FKBP‑tagged ORF4 and 48". Molecular Medicine Reports 17, no. 1 (2018): 763-770. https://doi.org/10.3892/mmr.2017.7986