NF‑κB regulates HSF1 and c‑Jun activation in heat stress‑induced intestinal epithelial cell apoptosis

  • Authors:
    • Jun Li
    • Yanan Liu
    • Pengkai Duan
    • Rongguo Yu
    • Zhengtao Gu
    • Li Li
    • Zhifeng Liu
    • Lei Su
  • View Affiliations

  • Published online on: December 6, 2017     https://doi.org/10.3892/mmr.2017.8199
  • Pages: 3388-3396
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Abstract

Heat stress may induce intestinal epithelial cell apoptosis; however, the molecular mechanisms have not yet been identified. The present study used IEC‑6 rat small intestinal epithelial cells to investigate heat stress‑induced production of reactive oxygen species (ROS), which may be involved in nuclear factor (NF)‑κB activation during heat stress. IEC‑6 cells were transfected with NF‑κB p65‑specific small interfering RNA (siRNA), and observed a significant increase in cell apoptosis and caspase‑3 cleavage; however, in cells transfected with adenovirus that constitutively overexpressed p65, the opposite results were obtained. Furthermore, p65 knockdown increased the heat stress‑induced expression and activity of heat shock transcription factor 1 (HSF1); conversely, p65 overexpression slightly decreased HSF1 activity. The levels of heat stress‑induced c‑Jun phosphorylation were also examined: Knockdown of p65 resulted in a reduction of c‑Jun phosphorylation, whereas p65 overexpression resulted in increased phosphorylation. Furthermore, siRNA‑mediated knockdown of HSF1 in IEC‑6 cells significantly increased heat stress‑induced apoptosis. Cells pretreated with c‑Jun peptide, an inhibitor of c‑Jun activation, exhibited a significant reduction in apoptosis. These findings indicated that heat stress stimulation in IEC‑6 cells induced the pro‑apoptotic role of NF‑κB by regulating HSF1 and c‑Jun activation.
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February-2018
Volume 17 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Li J, Liu Y, Duan P, Yu R, Gu Z, Li L, Liu Z and Su L: NF‑κB regulates HSF1 and c‑Jun activation in heat stress‑induced intestinal epithelial cell apoptosis. Mol Med Rep 17: 3388-3396, 2018
APA
Li, J., Liu, Y., Duan, P., Yu, R., Gu, Z., Li, L. ... Su, L. (2018). NF‑κB regulates HSF1 and c‑Jun activation in heat stress‑induced intestinal epithelial cell apoptosis. Molecular Medicine Reports, 17, 3388-3396. https://doi.org/10.3892/mmr.2017.8199
MLA
Li, J., Liu, Y., Duan, P., Yu, R., Gu, Z., Li, L., Liu, Z., Su, L."NF‑κB regulates HSF1 and c‑Jun activation in heat stress‑induced intestinal epithelial cell apoptosis". Molecular Medicine Reports 17.2 (2018): 3388-3396.
Chicago
Li, J., Liu, Y., Duan, P., Yu, R., Gu, Z., Li, L., Liu, Z., Su, L."NF‑κB regulates HSF1 and c‑Jun activation in heat stress‑induced intestinal epithelial cell apoptosis". Molecular Medicine Reports 17, no. 2 (2018): 3388-3396. https://doi.org/10.3892/mmr.2017.8199