Open Access

Expression of immunoglobulin A in human mesangial cells and its effects on cell apoptosis and adhesion

  • Authors:
    • Hui Deng
    • Junfan Ma
    • Ziyang Jing
    • Zhenling Deng
    • Yaoxian Liang
    • Lata A
    • Yang Liu
    • Xiaoyan Qiu
    • Yue Wang
  • View Affiliations

  • Published online on: February 2, 2018     https://doi.org/10.3892/mmr.2018.8544
  • Pages: 5272-5282
  • Copyright: © Deng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

IgA nephropathy (IgAN) is characterized by predominant IgA deposition in the glomerular mesangium. It has been considered that the deposited IgA is synthesized by B cells, although recent reports have suggested the implication of other cell types. Therefore, the present study investigated whether glomerular mesangial cells could produce IgA by themselves. Semi‑quantitative reverse transcription-polymerase chain reaction, and immunostaining analysis revealed that the IgA protein and gene transcripts were expressed in primary human renal mesangial cells (HRMCs). Furthermore, the IgA heavy chain (α1 and α2) and the light chain (κ and λ) were localized in the cytoplasm or were located on the cell membranes of human mesangial cells (HMCs). Mass spectrometry results indicated that Ig α1 and Ig α2 were secreted in the culture media of HMCs. The transcripts of Ig α, Ig κ and Ig λ constant regions were detected. The predominant rearrangement pattern of the variable region of Ig κ, was Vκ3‑20*01/Jκ1*01 in HMCs and Vκ1‑12*01/Jκ4*01 in HRMCs. In addition, knockdown of Ig α1 expression by small interfering RNA (siRNA) inhibited cell adhesion and promoted apoptosis. Our findings demonstrate that HMCs can express IgA, and that this expression is associated with cell functions, which may contribute to the deposition of IgA in patients with IgAN.
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April-2018
Volume 17 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Deng H, Ma J, Jing Z, Deng Z, Liang Y, A L, Liu Y, Qiu X and Wang Y: Expression of immunoglobulin A in human mesangial cells and its effects on cell apoptosis and adhesion. Mol Med Rep 17: 5272-5282, 2018
APA
Deng, H., Ma, J., Jing, Z., Deng, Z., Liang, Y., A, L. ... Wang, Y. (2018). Expression of immunoglobulin A in human mesangial cells and its effects on cell apoptosis and adhesion. Molecular Medicine Reports, 17, 5272-5282. https://doi.org/10.3892/mmr.2018.8544
MLA
Deng, H., Ma, J., Jing, Z., Deng, Z., Liang, Y., A, L., Liu, Y., Qiu, X., Wang, Y."Expression of immunoglobulin A in human mesangial cells and its effects on cell apoptosis and adhesion". Molecular Medicine Reports 17.4 (2018): 5272-5282.
Chicago
Deng, H., Ma, J., Jing, Z., Deng, Z., Liang, Y., A, L., Liu, Y., Qiu, X., Wang, Y."Expression of immunoglobulin A in human mesangial cells and its effects on cell apoptosis and adhesion". Molecular Medicine Reports 17, no. 4 (2018): 5272-5282. https://doi.org/10.3892/mmr.2018.8544