Open Access

BAG3 promotes chondrosarcoma progression by upregulating the expression of β-catenin

  • Authors:
    • Huijuan Shi
    • Wenfang Chen
    • Yu Dong
    • Xiaofang Lu
    • Wenhui Zhang
    • Liantang Wang
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  • Published online on: February 15, 2018     https://doi.org/10.3892/mmr.2018.8611
  • Pages: 5754-5763
  • Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

To investigate the roles of B‑cell lymphoma‑2 associated athanogene 3 (BAG3) in human chondrosarcoma and the potential mechanisms, the expression levels of BAG3 were detected in the present study, and the associations between BAG3 and clinical pathological parameters, clinical stage as well as the survival of patients were analyzed. The present study detected BAG3 mRNA and protein expression in the normal cartilage cell line HC‑a and in SW1353 chondrosarcoma cells by reverse transcription‑quantitative polymerase chain reaction and western blot analysis. The BAG3 protein expression in 59 cases of chondrosarcoma, 30 patients with endogenous chondroma and 8 cases of normal cartilage was semi-quantitatively analyzed using the immunohistochemical method. In addition, the BAG3 protein expression level, the clinical pathological parameters, clinical stage and the survival time of patients with chondrosarcoma were analyzed. The plasmid transfection method was employed to upregulate the expression BAG3 and small RNA interference to downregulate the expression of BAG3 in SW1353 cells. The expression levels of BAG3 protein and mRNA were significantly increased in the chondrosarcoma cell line when compared with the normal cartilage cell line. The immunohistochemistry results indicated that BAG3 protein was overexpressed in the tissue of human chondrosarcoma. Statistical analysis showed that the expression level of BAG3 was significantly increased in the different Enneking staging of patients with chondrosarcoma and Tumor staging, and there were no statistical differences in age, gender, histological classification and tumor size. In the in vitro experiments, the data revealed that BAG3 significantly promoted chondrosarcoma cell proliferation, colony‑formation, migration and invasion; however, it inhibited chondrosarcoma cell apoptosis. It was observed that BAG3 upregulated β‑catenin expression at the mRNA and protein levels. In addition, BAG3 induced the expression of runt‑related transcription factor 2 (RUNX2) in chondrosarcoma cells by upregulating β‑catenin. These clinical analyses revealed a positive association between β‑catenin and BAG3 in chondrosarcoma tumors. BAG3 was significantly increased in chondrosarcoma cells and tissues compared with the normal cartilage cells, tissue and cartilage benign tumors. Thus, BAG3 may serve as an oncogene in the development of chondrosarcoma via the induction of RUNX2 expression. The results of the present study contribute to further research on the biological development of chondrosarcoma.
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April-2018
Volume 17 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Shi H, Chen W, Dong Y, Lu X, Zhang W and Wang L: BAG3 promotes chondrosarcoma progression by upregulating the expression of β-catenin. Mol Med Rep 17: 5754-5763, 2018
APA
Shi, H., Chen, W., Dong, Y., Lu, X., Zhang, W., & Wang, L. (2018). BAG3 promotes chondrosarcoma progression by upregulating the expression of β-catenin. Molecular Medicine Reports, 17, 5754-5763. https://doi.org/10.3892/mmr.2018.8611
MLA
Shi, H., Chen, W., Dong, Y., Lu, X., Zhang, W., Wang, L."BAG3 promotes chondrosarcoma progression by upregulating the expression of β-catenin". Molecular Medicine Reports 17.4 (2018): 5754-5763.
Chicago
Shi, H., Chen, W., Dong, Y., Lu, X., Zhang, W., Wang, L."BAG3 promotes chondrosarcoma progression by upregulating the expression of β-catenin". Molecular Medicine Reports 17, no. 4 (2018): 5754-5763. https://doi.org/10.3892/mmr.2018.8611