Tumor necrosis factor-α-induced protein 8-like-2 is involved in the activation of macrophages by Astragalus polysaccharides in vitro

  • Authors:
    • Jie Zhu
    • Yuanyuan Zhang
    • Fanghua Fan
    • Guoyou Wu
    • Zhen Xiao
    • Huanqin Zhou
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  • Published online on: March 13, 2018     https://doi.org/10.3892/mmr.2018.8730
  • Pages: 7428-7434
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Abstract

In previous years, studies have shown that Astragalus polysaccharides (APS) can improve cellular immunity and humoral immune function, which has become a focus of investigations. Tumor necrosis factor‑α‑induced protein 8‑like 2 (TIPE2) is a negative regulator of immune reactions. However, the effect and underlying mechanisms of TIPE2 on the APS‑induced immune response remains to be fully elucidated. The present study aimed to examine the role of TIPE2 and its underlying mechanisms in the APS‑induced immune response. The production of nitric oxide (NO) was detected in macrophages in vitro following APS stimulation. In addition, the present study interfered with the expression of TIPE2 in macrophages, and examined the production of cytokines, NO and components of the mitogen‑activate protein kinase (MAPK) signaling pathway following APS stimulation. The results showed that APS was able to activate macrophages by inducing the production of interleukin (IL)‑1β, tumor necrosis factor (TNF)‑α, IL‑6 and NO. Furthermore, RAW264.7 cells were stimulated with APS when TIPE2 was silenced, and it was found that the production of TNF‑α, IL‑6, IL‑1β and NO were upregulated, and the signaling pathway of MAPK was activated. Taken together, these results demonstrated that TIPE2 had an important negative effect on the APS‑induced production of inflammatory cytokines and NO via the MAPK signaling pathway.
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May-2018
Volume 17 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhu J, Zhang Y, Fan F, Wu G, Xiao Z and Zhou H: Tumor necrosis factor-α-induced protein 8-like-2 is involved in the activation of macrophages by Astragalus polysaccharides in vitro. Mol Med Rep 17: 7428-7434, 2018
APA
Zhu, J., Zhang, Y., Fan, F., Wu, G., Xiao, Z., & Zhou, H. (2018). Tumor necrosis factor-α-induced protein 8-like-2 is involved in the activation of macrophages by Astragalus polysaccharides in vitro. Molecular Medicine Reports, 17, 7428-7434. https://doi.org/10.3892/mmr.2018.8730
MLA
Zhu, J., Zhang, Y., Fan, F., Wu, G., Xiao, Z., Zhou, H."Tumor necrosis factor-α-induced protein 8-like-2 is involved in the activation of macrophages by Astragalus polysaccharides in vitro". Molecular Medicine Reports 17.5 (2018): 7428-7434.
Chicago
Zhu, J., Zhang, Y., Fan, F., Wu, G., Xiao, Z., Zhou, H."Tumor necrosis factor-α-induced protein 8-like-2 is involved in the activation of macrophages by Astragalus polysaccharides in vitro". Molecular Medicine Reports 17, no. 5 (2018): 7428-7434. https://doi.org/10.3892/mmr.2018.8730