Open Access

Association between gastrointestinal motility and macrophage/mast cell distribution in mice during the healing stage after DSS‑induced colitis

  • Authors:
    • Mio Kodani
    • Hirokazu Fukui
    • Toshihiko Tomita
    • Tadayuki Oshima
    • Jiro Watari
    • Hiroto Miwa
  • View Affiliations

  • Published online on: April 24, 2018     https://doi.org/10.3892/mmr.2018.8926
  • Pages: 8167-8172
  • Copyright : © Kodani et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

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Abstract

Irritable bowel syndrome (IBS) frequently occurs after infectious colitis or inflammatory bowel disease in patients with complete remission. This suggests that post‑inflammation‑associated factors may serve a role in the pathophysiology of IBS; however, the mechanism responsible remains unclear. In the present study, the involvement of macrophages and mast cells in alteration of gastrointestinal (GI) motility was investigated in mice in the remission stage after acute colitis. C57BL/6 mice were administered 2% dextran sulfate sodium in drinking water for 5 days and their intestinal tissues were investigated at intervals for up to 24 weeks. Expression of the mannose receptor (MR) and tryptase was examined by immunohistochemistry, and the GI transit time (GITT) was measured by administration of carmine red solution. A minimal degree of inflammatory cell infiltration persisted in the colon and also the small intestine of mice in remission after colitis and the GITT was significantly shorter. The number of muscularis MR‑positive macrophages was significantly increased in the small intestine of mice in remission after colitis and negatively correlated with GITT. Furthermore, results indicated that the number of muscularis tryptase‑positive mast cells was significantly increased throughout the intestine of mice during the healing process after colitis and was positively correlated with GITT. The present findings suggested an increased number of macrophages and/or mast cells in the intestinal muscular layer may be associated with the pathophysiology of GI dysmotility after colitis.
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June-2018
Volume 17 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Kodani M, Fukui H, Tomita T, Oshima T, Watari J and Miwa H: Association between gastrointestinal motility and macrophage/mast cell distribution in mice during the healing stage after DSS‑induced colitis. Mol Med Rep 17: 8167-8172, 2018
APA
Kodani, M., Fukui, H., Tomita, T., Oshima, T., Watari, J., & Miwa, H. (2018). Association between gastrointestinal motility and macrophage/mast cell distribution in mice during the healing stage after DSS‑induced colitis. Molecular Medicine Reports, 17, 8167-8172. https://doi.org/10.3892/mmr.2018.8926
MLA
Kodani, M., Fukui, H., Tomita, T., Oshima, T., Watari, J., Miwa, H."Association between gastrointestinal motility and macrophage/mast cell distribution in mice during the healing stage after DSS‑induced colitis". Molecular Medicine Reports 17.6 (2018): 8167-8172.
Chicago
Kodani, M., Fukui, H., Tomita, T., Oshima, T., Watari, J., Miwa, H."Association between gastrointestinal motility and macrophage/mast cell distribution in mice during the healing stage after DSS‑induced colitis". Molecular Medicine Reports 17, no. 6 (2018): 8167-8172. https://doi.org/10.3892/mmr.2018.8926