Open Access

Overexpression of retinoblastoma‑binding protein 4 contributes to the radiosensitivity of AGS gastric cancer cells via phosphoinositide3‑kinase/protein kinase B pathway suppression

  • Authors:
    • Xiaoxi Jin
    • Rui Jiang
    • Yongsheng Xiang
    • Zhen Fan
    • Zhiwei Wu
    • Bo Yang
    • Lujun Yang
    • Shanshan Wei
    • Yan Yang
  • View Affiliations

  • Published online on: June 11, 2018     https://doi.org/10.3892/mmr.2018.9153
  • Pages: 1571-1581
  • Copyright: © Jin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In the present study, the effects and underlying mechanism of RbAp48 on the radiosensitivity of AGS gastric cancer cells was investigated. Cell proliferation was determined with an MTT assay. Flow cytometry was performed to evaluate the cell cycle and apoptosis. Reverse transcription‑quantitative polymerase chain reaction and western blot analysis were performed to detect mRNA and protein expression, respectively, including RbAp48, phosphoinositide 3‑kinase (PI3K) and protein kinase B (Akt). The results revealed that radiation enhanced the expression level of RbAp48 in AGS cells, and that RbAp48 combined with radiation reduced AGS cell proliferation. In addition, RbAp48 combined with radiation resulted in G2 phase arrest and induced apoptosis via regulation of the PI3K/Akt pathway. In conclusion, it was demonstrated that overexpression of RbAp48 enhanced the radiosensitivity of AGS gastric cancer cells via suppression of PI3K/Akt pathway activity, suggesting that RbAp48 may hold potential as a gene therapeutic strategy in the future, aiding in the treatment of gastric cancer.
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August-2018
Volume 18 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Jin X, Jiang R, Xiang Y, Fan Z, Wu Z, Yang B, Yang L, Wei S and Yang Y: Overexpression of retinoblastoma‑binding protein 4 contributes to the radiosensitivity of AGS gastric cancer cells via phosphoinositide3‑kinase/protein kinase B pathway suppression. Mol Med Rep 18: 1571-1581, 2018
APA
Jin, X., Jiang, R., Xiang, Y., Fan, Z., Wu, Z., Yang, B. ... Yang, Y. (2018). Overexpression of retinoblastoma‑binding protein 4 contributes to the radiosensitivity of AGS gastric cancer cells via phosphoinositide3‑kinase/protein kinase B pathway suppression. Molecular Medicine Reports, 18, 1571-1581. https://doi.org/10.3892/mmr.2018.9153
MLA
Jin, X., Jiang, R., Xiang, Y., Fan, Z., Wu, Z., Yang, B., Yang, L., Wei, S., Yang, Y."Overexpression of retinoblastoma‑binding protein 4 contributes to the radiosensitivity of AGS gastric cancer cells via phosphoinositide3‑kinase/protein kinase B pathway suppression". Molecular Medicine Reports 18.2 (2018): 1571-1581.
Chicago
Jin, X., Jiang, R., Xiang, Y., Fan, Z., Wu, Z., Yang, B., Yang, L., Wei, S., Yang, Y."Overexpression of retinoblastoma‑binding protein 4 contributes to the radiosensitivity of AGS gastric cancer cells via phosphoinositide3‑kinase/protein kinase B pathway suppression". Molecular Medicine Reports 18, no. 2 (2018): 1571-1581. https://doi.org/10.3892/mmr.2018.9153