Open Access

‘Heart development and morphogenesis’ is a novel pathway for human ovarian granulosa cell differentiation during long‑term in vitro cultivation‑a microarray approach

  • Authors:
    • Wiesława Kranc
    • Maciej Brązert
    • Piotr Celichowski
    • Artur Bryja
    • Mariusz J. Nawrocki
    • Katarzyna Ożegowska
    • Maurycy Jankowski
    • Michal Jeseta
    • Leszek Pawelczyk
    • Andrzej Bręborowicz
    • Dominik Rachoń
    • Mariusz T. Skowroński
    • Małgorzata Bruska
    • Maciej Zabel
    • Michał Nowicki
    • Bartosz Kempisty
  • View Affiliations

  • Published online on: January 8, 2019     https://doi.org/10.3892/mmr.2019.9837
  • Pages: 1705-1715
  • Copyright: © Kranc et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Granulosa cells (GCs) have many functions in the endocrine system. Most notably, they produce progesterone following ovulation. However, it has recently been proven that GCs can change their properties when subjected to long‑term culture. In the present study, GCs were collected from hyper‑stimulated ovarian follicles during in vitro fertilization procedures. They were grown in vitro, in a long‑term manner. RNA was collected following 1, 7, 15 and 30 days of culture. Expression microarrays were used for analysis, which allowed to identify groups of genes characteristic for particular cellular processes. In addition, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was performed to validate the obtained results. Two ontological groups characteristic for processes associated with the development and morphogenesis of the heart were identified during the analyses: ‘Heart development’ and ‘heart morphogenesis’. The results of the microarrays revealed that the highest change in expression was demonstrated by the lysyl Oxidase, oxytocin receptor, nexilin F‑actin binding protein, and cysteine‑rich protein 3 genes. The lowest change was exhibited by odd‑skipped related transcription factor 1, plakophilin 2, transcription growth factor‑β receptor 1, and kinesin family member 3A. The direction of changes was confirmed by RT‑qPCR results. In the present study, it was suggested that GCs may have the potential to differentiate towards other cell types under long‑term in vitro culture conditions. Thus, genes belonging to the presented ontological groups can be considered as novel markers of proliferation and differentiation of GCs towards the heart muscle cells.
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March-2019
Volume 19 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Kranc W, Brązert M, Celichowski P, Bryja A, Nawrocki MJ, Ożegowska K, Jankowski M, Jeseta M, Pawelczyk L, Bręborowicz A, Bręborowicz A, et al: ‘Heart development and morphogenesis’ is a novel pathway for human ovarian granulosa cell differentiation during long‑term in vitro cultivation‑a microarray approach. Mol Med Rep 19: 1705-1715, 2019
APA
Kranc, W., Brązert, M., Celichowski, P., Bryja, A., Nawrocki, M.J., Ożegowska, K. ... Kempisty, B. (2019). ‘Heart development and morphogenesis’ is a novel pathway for human ovarian granulosa cell differentiation during long‑term in vitro cultivation‑a microarray approach. Molecular Medicine Reports, 19, 1705-1715. https://doi.org/10.3892/mmr.2019.9837
MLA
Kranc, W., Brązert, M., Celichowski, P., Bryja, A., Nawrocki, M. J., Ożegowska, K., Jankowski, M., Jeseta, M., Pawelczyk, L., Bręborowicz, A., Rachoń, D., Skowroński, M. T., Bruska, M., Zabel, M., Nowicki, M., Kempisty, B."‘Heart development and morphogenesis’ is a novel pathway for human ovarian granulosa cell differentiation during long‑term in vitro cultivation‑a microarray approach". Molecular Medicine Reports 19.3 (2019): 1705-1715.
Chicago
Kranc, W., Brązert, M., Celichowski, P., Bryja, A., Nawrocki, M. J., Ożegowska, K., Jankowski, M., Jeseta, M., Pawelczyk, L., Bręborowicz, A., Rachoń, D., Skowroński, M. T., Bruska, M., Zabel, M., Nowicki, M., Kempisty, B."‘Heart development and morphogenesis’ is a novel pathway for human ovarian granulosa cell differentiation during long‑term in vitro cultivation‑a microarray approach". Molecular Medicine Reports 19, no. 3 (2019): 1705-1715. https://doi.org/10.3892/mmr.2019.9837