Open Access

Expression and significance of EBV, ARID1A and PIK3CA in gastric carcinoma

  • Authors:
    • Huan Zhou
    • Shun Tan
    • Hong Li
    • Xiangtao Lin
  • View Affiliations

  • Published online on: January 22, 2019     https://doi.org/10.3892/mmr.2019.9886
  • Pages: 2125-2136
  • Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

AT‑rich interaction domain 1A (ARID1A) and phosphatidylinositol‑4,5‑bisphosphate 3‑kinase catalytic subunit α (PIK3CA) serve important roles in the formation and development of numerous malignancies including gastric cancer. Accumulating evidence has demonstrated that Epstein‑Barr virus (EBV) is a pathogenic virus associated with gastric cancer. The present study aimed to investigate the association between EBV infection, and the expression levels of ARID1A and PIK3CA in gastric cancer. EBER in situ hybridization was performed to detect EBV infection. Immunohistochemistry was used to assess the expression levels of ARID1A and PIK3CA in gastric cancer and adjacent normal tissues. A total of 58 gastric cancer and 10 adjacent normal tissues were tested for genetic mutations via single nucleotide polymorphism genotyping assays. Fluorescent polymerase chain reaction was used to detect EBV infection; 9.3% (28/300) of gastric cancer samples were positive for EBV, whereas, all adjacent normal tissues were negative. ARID1A and PIK3CA were negatively correlated in gastric cancer (r=‑0.167). The expression levels of ARID1A and PIK3CA in gastric cancer were significantly associated with the depth of invasion of gastric cancer. A total of 62.1% (36/58) of tumor samples exhibited mutations in ARID1A, whereas, 13.8% (8/58) presented mutations in PIK3CA. Notably, EBV‑associated gastric cancer (EBVaGC) samples with PIK3CA mutations additionally exhibited ARID1A mutations. Although in the present study it was identified that ARID1A and PIK3CA were negatively correlated in EBVaGC, further studies are required to investigate the association among ARID1A, PIK3CA and EBV in gastric cancer.
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March-2019
Volume 19 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhou H, Tan S, Li H and Lin X: Expression and significance of EBV, ARID1A and PIK3CA in gastric carcinoma. Mol Med Rep 19: 2125-2136, 2019
APA
Zhou, H., Tan, S., Li, H., & Lin, X. (2019). Expression and significance of EBV, ARID1A and PIK3CA in gastric carcinoma. Molecular Medicine Reports, 19, 2125-2136. https://doi.org/10.3892/mmr.2019.9886
MLA
Zhou, H., Tan, S., Li, H., Lin, X."Expression and significance of EBV, ARID1A and PIK3CA in gastric carcinoma". Molecular Medicine Reports 19.3 (2019): 2125-2136.
Chicago
Zhou, H., Tan, S., Li, H., Lin, X."Expression and significance of EBV, ARID1A and PIK3CA in gastric carcinoma". Molecular Medicine Reports 19, no. 3 (2019): 2125-2136. https://doi.org/10.3892/mmr.2019.9886