Open Access

TGF‑β1 promotes the osteoinduction of human osteoblasts via the PI3K/AKT/mTOR/S6K1 signalling pathway

  • Authors:
    • Zhaodong Zhang
    • Xiuzhi Zhang
    • Dewei Zhao
    • Baoyi Liu
    • Benjie Wang
    • Weiting Yu
    • Junlei Li
    • Xiaobing Yu
    • Fang Cao
    • Guoshuang Zheng
    • Yao Zhang
    • Yupeng Liu
  • View Affiliations

  • Published online on: March 18, 2019     https://doi.org/10.3892/mmr.2019.10051
  • Pages: 3505-3518
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Transforming growth factor β1 (TGF‑β1) has been suggested to be a candidate cytokine in the field of bone tissue engineering. Cytokines serve important roles in tissue engineering, particularly in the repair of bone damage; however, the underlying molecular mechanisms remain unclear. In the present study, the effects of TGF‑β1 on the osteogenesis and motility of hFOB1.19 human osteoblasts were demonstrated via the phenotype and gene expression of cells. Additionally, the role of the phosphatidylinositol 3‑kinase/protein kinase B/mammalian target of rapamycin/S6 kinase 1 (PI3K/AKT/mTOR/S6K1) signalling pathway in the effects of TGF‑β1 on osteoblasts was investigated. It was demonstrated using Cell Counting Kit‑8 and flow cytometry assays that the proliferation of human osteoblasts was promoted by 1 ng/ml TGF‑β1. In addition, alkaline phosphatase activity, Alizarin red staining, scratch‑wound and Transwell assays were conducted. It was revealed that osteogenesis and the migration of cells were regulated by TGF‑β1 via the upregulation of osteogenic and migration‑associated genes. Alterations in the expression of osteogenesis‑ and migration‑associated genes were evaluated following pre‑treatment with a PI3K/AKT inhibitor (LY294002) and an mTOR/S6K1 inhibitor (rapamycin), with or without TGF‑β1. The results indicated that TGF‑β1 affected the osteogenesis and mineralisation of osteoblasts via the PI3K/AKT signalling pathway. Furthermore, TGF‑β1 exhibited effects on mTOR/S6K1 downstream of PI3K/AKT. The present study demonstrated that TGF‑β1 promoted the proliferation, differentiation and migration of human hFOB1.19 osteoblasts, and revealed that TGF‑β1 affected the biological activity of osteoblasts via the PI3K/AKT/mTOR/S6K1 signalling pathway. Our findings may provide novel insight to aid the development of bone tissue engineering methods for the treatment of bone injury.
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May-2019
Volume 19 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Zhang Z, Zhang X, Zhao D, Liu B, Wang B, Yu W, Li J, Yu X, Cao F, Zheng G, Zheng G, et al: TGF‑β1 promotes the osteoinduction of human osteoblasts via the PI3K/AKT/mTOR/S6K1 signalling pathway. Mol Med Rep 19: 3505-3518, 2019
APA
Zhang, Z., Zhang, X., Zhao, D., Liu, B., Wang, B., Yu, W. ... Liu, Y. (2019). TGF‑β1 promotes the osteoinduction of human osteoblasts via the PI3K/AKT/mTOR/S6K1 signalling pathway. Molecular Medicine Reports, 19, 3505-3518. https://doi.org/10.3892/mmr.2019.10051
MLA
Zhang, Z., Zhang, X., Zhao, D., Liu, B., Wang, B., Yu, W., Li, J., Yu, X., Cao, F., Zheng, G., Zhang, Y., Liu, Y."TGF‑β1 promotes the osteoinduction of human osteoblasts via the PI3K/AKT/mTOR/S6K1 signalling pathway". Molecular Medicine Reports 19.5 (2019): 3505-3518.
Chicago
Zhang, Z., Zhang, X., Zhao, D., Liu, B., Wang, B., Yu, W., Li, J., Yu, X., Cao, F., Zheng, G., Zhang, Y., Liu, Y."TGF‑β1 promotes the osteoinduction of human osteoblasts via the PI3K/AKT/mTOR/S6K1 signalling pathway". Molecular Medicine Reports 19, no. 5 (2019): 3505-3518. https://doi.org/10.3892/mmr.2019.10051