Nonactin hinders intracellular glycosylation in virus-infected baby hamster kidney cells

  • Authors:
    • Jin-Man Lee
    • Jong-Guk Kim
    • Tae-Ho Kim
    • Do S. Lee
    • Jae H. Kim
    • Somi K. Cho
    • Key Z. Riu
    • Dong-Sun Lee
    • Sang-Han Lee
  • View Affiliations

  • Published online on: January 1, 2010     https://doi.org/10.3892/mmr_00000227
  • Pages: 115-119
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Abstract

Potent antiviral agents hinder virus-infected cell machinery, leading to rescue from viral damage. In this study, we aimed to identify selective intracellular glycosylation inhibitor(s) that do not suppress glycoprotein synthesis. Our results showed that nonactin is a potent inhibitor of intracellular glycosylation. First, we examined the effects of nonactin on syncytium formation and cytopathic activity in virus-infected baby hamster kidney cells. Nonactin effectively inhibited syncytium formation in a concentration-dependent manner, and infectious virus production was markedly reduced. However, glycoprotein synthesis was not affected. In the presence of 5 µg/ml nonactin, we observed the intracellular accumulation of vesicular stomatitis virus-G protein as well as syncytium formation, but no significant effects on Newcastle disease virus-hemagglutinin-neuramidase glycoprotein synthesis. Our results collectively indicate that nonactin potentially inhibits glycosylation by acting as a suppressor of intracellular glycosylation trafficking.

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January-February 2010
Volume 3 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Lee J, Kim J, Kim T, Lee DS, Kim JH, Cho SK, Riu KZ, Lee D and Lee S: Nonactin hinders intracellular glycosylation in virus-infected baby hamster kidney cells . Mol Med Rep 3: 115-119, 2010
APA
Lee, J., Kim, J., Kim, T., Lee, D.S., Kim, J.H., Cho, S.K. ... Lee, S. (2010). Nonactin hinders intracellular glycosylation in virus-infected baby hamster kidney cells . Molecular Medicine Reports, 3, 115-119. https://doi.org/10.3892/mmr_00000227
MLA
Lee, J., Kim, J., Kim, T., Lee, D. S., Kim, J. H., Cho, S. K., Riu, K. Z., Lee, D., Lee, S."Nonactin hinders intracellular glycosylation in virus-infected baby hamster kidney cells ". Molecular Medicine Reports 3.1 (2010): 115-119.
Chicago
Lee, J., Kim, J., Kim, T., Lee, D. S., Kim, J. H., Cho, S. K., Riu, K. Z., Lee, D., Lee, S."Nonactin hinders intracellular glycosylation in virus-infected baby hamster kidney cells ". Molecular Medicine Reports 3, no. 1 (2010): 115-119. https://doi.org/10.3892/mmr_00000227