Analysis of invasion-metastasis in pancreatic cancer: Correlation between the expression and arrangement of tight junction protein-2 and cell dissociation in pancreatic cancer cells

  • Authors:
    • Lei Zhou
    • Xiaodong Tan
    • Wei Wang
    • Baosheng Wang
    • Xianwei Dai
    • Jingang Liu
  • View Affiliations

  • Published online on: January 1, 2010     https://doi.org/10.3892/mmr_00000232
  • Pages: 149-153
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Abstract

High frequency of invasion and metastasis is one of the key characteristics of pancreatic cancer. In our recent study, tight junction protein-2 (Tjp-2) was identified as a differentially expressed gene related to invasion-metastasis in highly (PC-1.0) and weakly (PC-1) invasive and metastatic pancreatic cancer cells by cDNA microarray analysis. Changes in the structure and function of tight junctions are correlated with carcinogenesis and tumour development. In this study, RT-PCR, Western blotting and immunocytochemistry were used to study the correlation between the expression and localisation of Tjp-2 and cell dissociation in pancreatic cancer. Tjp-2 mRNA and protein were differentially expressed in PC-1.0 and PC-1 cells. Furthermore, the addition of dissociation factor (DF) or U0126 (a MEK inhibitor) significantly induced changes in the mRNA expression and protein intracellular localisation of Tjp-2, and in the simultaneous cell dissociation of PC-1.0 and PC-1 cells. However, protein expression of Tjp-2 was not affected by DF or U0126 treatment. The current results indicate that Tjp-2 is involved in the regulation of cell dissociation in pancreatic cancer cells through changes in gene expression and intracellular localisation. Tjp-2 may serve as a new target for molecular therapies that prevent the invasion and metastasis of pancreatic cancer.

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January-February 2010
Volume 3 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhou L, Tan X, Wang W, Wang B, Dai X and Liu J: Analysis of invasion-metastasis in pancreatic cancer: Correlation between the expression and arrangement of tight junction protein-2 and cell dissociation in pancreatic cancer cells . Mol Med Rep 3: 149-153, 2010
APA
Zhou, L., Tan, X., Wang, W., Wang, B., Dai, X., & Liu, J. (2010). Analysis of invasion-metastasis in pancreatic cancer: Correlation between the expression and arrangement of tight junction protein-2 and cell dissociation in pancreatic cancer cells . Molecular Medicine Reports, 3, 149-153. https://doi.org/10.3892/mmr_00000232
MLA
Zhou, L., Tan, X., Wang, W., Wang, B., Dai, X., Liu, J."Analysis of invasion-metastasis in pancreatic cancer: Correlation between the expression and arrangement of tight junction protein-2 and cell dissociation in pancreatic cancer cells ". Molecular Medicine Reports 3.1 (2010): 149-153.
Chicago
Zhou, L., Tan, X., Wang, W., Wang, B., Dai, X., Liu, J."Analysis of invasion-metastasis in pancreatic cancer: Correlation between the expression and arrangement of tight junction protein-2 and cell dissociation in pancreatic cancer cells ". Molecular Medicine Reports 3, no. 1 (2010): 149-153. https://doi.org/10.3892/mmr_00000232