Effect of membrane toxin 12 isolated from Naja naja atra on proliferation and invasion of human bladder cancer EJ cells

  • Authors:
    • Delin Yang
    • Jiansong Wang
    • Jun Li
    • Haifeng Wang
    • Huihai  He
    • Chao Zhang
    • Kai Wang
    • Hongyi Xu
  • View Affiliations

  • Published online on: October 11, 2011     https://doi.org/10.3892/mmr.2011.622
  • Pages: 266-269
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Abstract

We investigated the effect of membrane toxin 12 (MT-12), isolated from Naja naja atra, a species of Chinese Cobra, on the proliferation and invasion of human bladder cancer EJ cells and studied its mechanisms using MTT assay, Transwell chamber invasion assay, scanning electron microscopy and flow cytometry. The results indicated that MT-12 inhibited the proliferation of bladder cancer cells in a dose‑dependent manner. The half maximal inhibitory concentration (IC50) after 72 h was 0.66 µg/ml. The invasion of cells decreased with increasing doses of MT-12 (0.125-0.5 µg/ml, P<0.001). Expression of CXCR4 decreased with the effect of MT-12 for a particular concentration range (0.125-0.5 µg/ml). To conclude, MT-12 is capable of inhibiting the proliferation and invasion of bladder cancer EJ cells. This mechanism may be associated with reduced expression of CXCR4 protein.

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January 2012
Volume 5 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Yang D, Wang J, Li J, Wang H, He H, Zhang C, Wang K and Xu H: Effect of membrane toxin 12 isolated from Naja naja atra on proliferation and invasion of human bladder cancer EJ cells. Mol Med Rep 5: 266-269, 2012
APA
Yang, D., Wang, J., Li, J., Wang, H., He, H., Zhang, C. ... Xu, H. (2012). Effect of membrane toxin 12 isolated from Naja naja atra on proliferation and invasion of human bladder cancer EJ cells. Molecular Medicine Reports, 5, 266-269. https://doi.org/10.3892/mmr.2011.622
MLA
Yang, D., Wang, J., Li, J., Wang, H., He, H., Zhang, C., Wang, K., Xu, H."Effect of membrane toxin 12 isolated from Naja naja atra on proliferation and invasion of human bladder cancer EJ cells". Molecular Medicine Reports 5.1 (2012): 266-269.
Chicago
Yang, D., Wang, J., Li, J., Wang, H., He, H., Zhang, C., Wang, K., Xu, H."Effect of membrane toxin 12 isolated from Naja naja atra on proliferation and invasion of human bladder cancer EJ cells". Molecular Medicine Reports 5, no. 1 (2012): 266-269. https://doi.org/10.3892/mmr.2011.622