Microarray analysis of microRNA expression patterns in the semen of infertile men with semen abnormalities

  • Authors:
    • Te Liu
    • Weiwei Cheng
    • Yongtao Gao
    • Hui Wang
    • Zhixue Liu
  • View Affiliations

  • Published online on: June 25, 2012     https://doi.org/10.3892/mmr.2012.967
  • Pages: 535-542
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Abstract

microRNAs (miRNAs) play a crucial role in tissue development and the pathology of many diseases, however, the effects and roles of miRNAs in the development of semen abnormalities in infertile males have not yet been investigated. In this study, we analyzed and compared the miRNA expression profiles of abnormal semen from 86 infertile males with normal semen from 86 healthy males using an miRNA microarray. In total, 52 miRNAs were differentially expressed between the abnormal semen of infertile males and the normal semen of healthy males. The differential expression of selected miRNAs was validated by real time qRT-PCR and northern blotting: miR-574-5p, miR-297, miR-122, miR-1275, miR-373, miR-185 and miR-193b were upregulated (fold change >1.5, p<0.001) and miR-100, miR-512-3p, miR-16, miR-19b, miR-23b and miR-26a were downregulated (fold change <0.667, p<0.001) in the semen of infertile males with semen abnormalities. In conclusion, this study provides new insights into specific miRNAs that are associated with semen abnormalities in infertile males.
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September 2012
Volume 6 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Liu T, Cheng W, Gao Y, Wang H and Liu Z: Microarray analysis of microRNA expression patterns in the semen of infertile men with semen abnormalities. Mol Med Rep 6: 535-542, 2012
APA
Liu, T., Cheng, W., Gao, Y., Wang, H., & Liu, Z. (2012). Microarray analysis of microRNA expression patterns in the semen of infertile men with semen abnormalities. Molecular Medicine Reports, 6, 535-542. https://doi.org/10.3892/mmr.2012.967
MLA
Liu, T., Cheng, W., Gao, Y., Wang, H., Liu, Z."Microarray analysis of microRNA expression patterns in the semen of infertile men with semen abnormalities". Molecular Medicine Reports 6.3 (2012): 535-542.
Chicago
Liu, T., Cheng, W., Gao, Y., Wang, H., Liu, Z."Microarray analysis of microRNA expression patterns in the semen of infertile men with semen abnormalities". Molecular Medicine Reports 6, no. 3 (2012): 535-542. https://doi.org/10.3892/mmr.2012.967