MicroRNA‑650 in a copy number‑variable region regulates the production of interleukin 6 in human osteosarcoma cells

  • Authors:
    • Jun Ho Yun
    • Sanghoon Moon
    • Heun‑Sik Lee
    • Mi Yeong Hwang
    • Yeon‑Jung Kim
    • Ho‑Yeong Yu
    • Youngsoo Kim
    • Bok‑Ghee Han
    • Bong‑Jo Kim
    • Jeong‑Min Kim
  • View Affiliations

  • Published online on: August 7, 2015     https://doi.org/10.3892/ol.2015.3581
  • Pages: 2603-2609
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Abstract

Copy number variation is a well‑known genetic variation. microRNAs (miRNAs/miRs) are non‑coding RNAs that mediate gene expression by regulating target mRNAs. In the present study, copy number deletions encompassing miRNA coding regions were investigated to determine the association between the deletion of miRNA and its phenotypic effects. A total of 38 human miRNAs in copy number variants were identified and miR‑650, which is functional in the human osteosarcoma MG‑63 cell line, was selected. Overexpression of miR‑650 decreased the expression of inhibitor of growth family member 4 (ING4) in the MG‑63 cells and increased interleukin (IL)6 transcription, as well as IL6 secretion in IL1B‑stimulated cells. Furthermore, miR‑650 downregulated the amount of nuclear factor of κ light polypeptide gene enhancer in B cells inhibitor α and increased the transcriptional activity of nuclear factor (NF)κB. Downregulation of ING4 also increased the production of IL6, similar to miR‑650 overexpression. Taken together, these data indicate that miR‑650 plays a significant role in the production of IL6 by regulating ING4 expression and NFκB signaling in IL1B‑stimulated MG‑63 osteosarcoma cells.
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October-2015
Volume 10 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yun JH, Moon S, Lee HS, Hwang MY, Kim YJ, Yu HY, Kim Y, Han BG, Kim BJ, Kim JM, Kim JM, et al: MicroRNA‑650 in a copy number‑variable region regulates the production of interleukin 6 in human osteosarcoma cells. Oncol Lett 10: 2603-2609, 2015
APA
Yun, J.H., Moon, S., Lee, H., Hwang, M.Y., Kim, Y., Yu, H. ... Kim, J. (2015). MicroRNA‑650 in a copy number‑variable region regulates the production of interleukin 6 in human osteosarcoma cells. Oncology Letters, 10, 2603-2609. https://doi.org/10.3892/ol.2015.3581
MLA
Yun, J. H., Moon, S., Lee, H., Hwang, M. Y., Kim, Y., Yu, H., Kim, Y., Han, B., Kim, B., Kim, J."MicroRNA‑650 in a copy number‑variable region regulates the production of interleukin 6 in human osteosarcoma cells". Oncology Letters 10.4 (2015): 2603-2609.
Chicago
Yun, J. H., Moon, S., Lee, H., Hwang, M. Y., Kim, Y., Yu, H., Kim, Y., Han, B., Kim, B., Kim, J."MicroRNA‑650 in a copy number‑variable region regulates the production of interleukin 6 in human osteosarcoma cells". Oncology Letters 10, no. 4 (2015): 2603-2609. https://doi.org/10.3892/ol.2015.3581