Downregulation of miR‑362‑5p inhibits proliferation, migration and invasion of human breast cancer MCF7 cells

  • Authors:
    • Fang Ni
    • Zhaohua Gui
    • Qiang Guo
    • Zhongqian Hu
    • Xinyi Wang
    • Danlei Chen
    • Siying Wang
  • View Affiliations

  • Published online on: December 3, 2015     https://doi.org/10.3892/ol.2015.3993
  • Pages: 1155-1160
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Abstract

An increasing number of studies have indicated that the deregulation of microRNAs (miRNAs) contributes to tumorigenesis and metastasis. In the present study, significant upregulation of miR‑362‑5p was identified in the breast cancer MDA‑MB‑231 and MCF7 cell lines compared with the control CCD‑1095Sk cell line. The inhibition of miR‑362‑5p was demonstrated to significantly inhibit the cell proliferation, migration and invasion of human breast cancer MCF7 cells. In addition, the knockdown of miR‑362‑5p induced G1 arrest and promoted apoptosis in the breast cancer cells. Mechanistic investigations confirmed that the tumor suppressor gene CYLD is a direct target of miR‑362‑5p. The ectopic expression of miR‑362‑5p represses CYLD expression, whereas miR‑362‑5p inhibitor treatment induces CYLD protein expression and decreases NF‑κB expression in the downstream signaling pathway. Thus, these findings may provide novel insights into the molecular mechanisms through which miR‑362‑5p regulates breast cancer cell proliferation, migration and invasion. This study also suggests that miR‑362‑5p may act as a novel potential therapeutic target for the treatment of breast cancer.
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February-2016
Volume 11 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Ni F, Gui Z, Guo Q, Hu Z, Wang X, Chen D and Wang S: Downregulation of miR‑362‑5p inhibits proliferation, migration and invasion of human breast cancer MCF7 cells. Oncol Lett 11: 1155-1160, 2016
APA
Ni, F., Gui, Z., Guo, Q., Hu, Z., Wang, X., Chen, D., & Wang, S. (2016). Downregulation of miR‑362‑5p inhibits proliferation, migration and invasion of human breast cancer MCF7 cells. Oncology Letters, 11, 1155-1160. https://doi.org/10.3892/ol.2015.3993
MLA
Ni, F., Gui, Z., Guo, Q., Hu, Z., Wang, X., Chen, D., Wang, S."Downregulation of miR‑362‑5p inhibits proliferation, migration and invasion of human breast cancer MCF7 cells". Oncology Letters 11.2 (2016): 1155-1160.
Chicago
Ni, F., Gui, Z., Guo, Q., Hu, Z., Wang, X., Chen, D., Wang, S."Downregulation of miR‑362‑5p inhibits proliferation, migration and invasion of human breast cancer MCF7 cells". Oncology Letters 11, no. 2 (2016): 1155-1160. https://doi.org/10.3892/ol.2015.3993