Regulation of C6 glioma cell migration by thymol

  • Authors:
    • Kang Pa Lee
    • Jai‑Eun Kim
    • Won‑Hwan Park
    • Heeok Hong
  • View Affiliations

  • Published online on: February 17, 2016     https://doi.org/10.3892/ol.2016.4237
  • Pages: 2619-2624
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Abstract

Tumor cell motility exhibits a crucial role in tumor development. Therefore, the present study aimed to investigate whether thymol could reduce C6 glioma cell migration. Cell viability was determined using the EZ-Cytox Cell Viability kit. The scratch wound healing and Boyden chamber assays were performed to test C6 glioma cell migration in the presence of fetal bovine serum (FBS). Additionally, the study investigated whether signaling proteins relevant to C6 glioma cell migration, i.e., extracellular signal-regulated kinases (ERK)1/2, protein kinase Cα (PKCα), matrix metallopeptidase (MMP)9 and MMP2, were affected by thymol treatment. Up to 30 µM, thymol did not alter cell viability, whereas 100 µM thymol induced the death of ~20% of the cells. Furthermore, thymol (30 µM) significantly reduced FBS-induced migration. In the FBS-stimulated C6 glioma cells, thymol (30 µM) suppressed PKCα and ERK1/2 phosphorylation. MMP9 and MMP2 production was also significantly reduced by treatment with 30 µM thymol in the C6 glioma cells. Taken together, these results indicate that thymol attenuates C6 glioma cell migration. Additionally, the study suggests that the effect of thymol on the FBS-induced migration of C6 glioma cells affects PKCα and ERK1/2 signaling, and suppresses MMP9 and MMP2 production.
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April-2016
Volume 11 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Lee KP, Kim JE, Park WH and Hong H: Regulation of C6 glioma cell migration by thymol. Oncol Lett 11: 2619-2624, 2016
APA
Lee, K.P., Kim, J., Park, W., & Hong, H. (2016). Regulation of C6 glioma cell migration by thymol. Oncology Letters, 11, 2619-2624. https://doi.org/10.3892/ol.2016.4237
MLA
Lee, K. P., Kim, J., Park, W., Hong, H."Regulation of C6 glioma cell migration by thymol". Oncology Letters 11.4 (2016): 2619-2624.
Chicago
Lee, K. P., Kim, J., Park, W., Hong, H."Regulation of C6 glioma cell migration by thymol". Oncology Letters 11, no. 4 (2016): 2619-2624. https://doi.org/10.3892/ol.2016.4237