Predicting associations between microRNAs and target genes in breast cancer by bioinformatics analyses

  • Authors:
    • Tianying Zheng
    • Xing Zhang
    • Yonggang Wang
    • Xiucui Yu
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  • Published online on: June 15, 2016     https://doi.org/10.3892/ol.2016.4731
  • Pages: 1067-1073
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Abstract

Breast cancer is the leading type of cancer among females. However, the association between microRNAs (miRNAs) and target genes in breast tumorigenesis is poorly studied. The original data set GSE26659 was downloaded from the Gene Expression Omnibus, and then the differentially expressed miRNAs among 77 breast cancer patients and 17 controls were identified using the Limma package in R software. Furthermore, breast cancer‑related differentially expressed miRNAs were selected from a human miRNA disease database and their target genes were selected from five miRNA databases. Then, functional analysis was performed for the target genes followed by construction of a miRNA‑target gene network. A total of 34 differentially expressed miRNAs were identified, including 13 breast cancer‑related miRNAs. Moreover, the target genes of the 13 miRNAs were significantly enriched in regulation of transcription (P=7.43E‑09) and pathways related to cancer (P=3.33E‑11). Finally, eight upregulated miRNAs (including hsa-miR-425) and five downregulated miRNAs (including hsa-miR-143, hsa‑miR‑145 and hsa-miR-125b) were identified in the miRNA‑target gene network. In conclusion, using bioinformatics approaches, we demonstrate that the changes in regulation of transcription and cancer pathways may play significant roles in the process of breast cancerogenesis. Differentially expressed miRNAs and their target genes may be new targets for breast cancer therapy.
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August-2016
Volume 12 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zheng T, Zhang X, Wang Y and Yu X: Predicting associations between microRNAs and target genes in breast cancer by bioinformatics analyses. Oncol Lett 12: 1067-1073, 2016
APA
Zheng, T., Zhang, X., Wang, Y., & Yu, X. (2016). Predicting associations between microRNAs and target genes in breast cancer by bioinformatics analyses. Oncology Letters, 12, 1067-1073. https://doi.org/10.3892/ol.2016.4731
MLA
Zheng, T., Zhang, X., Wang, Y., Yu, X."Predicting associations between microRNAs and target genes in breast cancer by bioinformatics analyses". Oncology Letters 12.2 (2016): 1067-1073.
Chicago
Zheng, T., Zhang, X., Wang, Y., Yu, X."Predicting associations between microRNAs and target genes in breast cancer by bioinformatics analyses". Oncology Letters 12, no. 2 (2016): 1067-1073. https://doi.org/10.3892/ol.2016.4731