Genome-wide analysis of DNA methylation in hepatoblastoma tissues

  • Authors:
    • Ximao Cui
    • Baihui Liu
    • Shan Zheng
    • Kuiran Dong
    • Rui Dong
  • View Affiliations

  • Published online on: June 28, 2016     https://doi.org/10.3892/ol.2016.4789
  • Pages: 1529-1534
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Abstract

DNA methylation has a crucial role in cancer biology. In the present study, a genome-wide analysis of DNA methylation in hepatoblastoma (HB) tissues was performed to verify differential methylation levels between HB and normal tissues. As alpha-fetoprotein (AFP) has a critical role in HB, AFP methylation levels were also detected using pyrosequencing. Normal and HB liver tissue samples (frozen tissue) were obtained from patients with HB. Genome‑wide analysis of DNA methylation in these tissues was performed using an Infinium HumanMethylation450 BeadChip, and the results were confirmed with reverse transcription‑quantitative polymerase chain reaction. The Infinium HumanMethylation450 BeadChip demonstrated distinctively less methylation in HB tissues than in non‑tumor tissues. In addition, methylation enrichment was observed in positions near the transcription start site of AFP, which exhibited lower methylation levels in HB tissues than in non‑tumor liver tissues. Lastly, a significant negative correlation was observed between AFP messenger RNA expression and DNA methylation percentage, using linear Pearson's R correlation coefficients. The present results demonstrate differential methylation levels between HB and normal tissues, and imply that aberrant methylation of AFP in HB could reflect HB development. Expansion of these findings could provide useful insight into HB biology.
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August-2016
Volume 12 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Cui X, Liu B, Zheng S, Dong K and Dong R: Genome-wide analysis of DNA methylation in hepatoblastoma tissues. Oncol Lett 12: 1529-1534, 2016
APA
Cui, X., Liu, B., Zheng, S., Dong, K., & Dong, R. (2016). Genome-wide analysis of DNA methylation in hepatoblastoma tissues. Oncology Letters, 12, 1529-1534. https://doi.org/10.3892/ol.2016.4789
MLA
Cui, X., Liu, B., Zheng, S., Dong, K., Dong, R."Genome-wide analysis of DNA methylation in hepatoblastoma tissues". Oncology Letters 12.2 (2016): 1529-1534.
Chicago
Cui, X., Liu, B., Zheng, S., Dong, K., Dong, R."Genome-wide analysis of DNA methylation in hepatoblastoma tissues". Oncology Letters 12, no. 2 (2016): 1529-1534. https://doi.org/10.3892/ol.2016.4789