Open Access

miR‑101 inhibits glioma cell invasion via the downregulation of COX‑2

  • Authors:
    • Chunyang Ma
    • Chuanyi Zheng
    • Enqi Bai
    • Kun Yang
  • View Affiliations

  • Published online on: August 3, 2016     https://doi.org/10.3892/ol.2016.4939
  • Pages: 2538-2544
  • Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Glioma is the most common type of primary tumor of the central nervous system. The present study aimed to demonstrate the role of miR-101 and cyclooxygenase‑2 (COX‑2) in the initiation and development of glioma. The expression of miR-101 and COX-2 in normal and malignant human glial cells and tissues was determined by western blotting and quantitative polymerase chain reaction analysis. The role of miR-101 on COX-2 expression was evaluated by a dual‑luciferase reporter assay. The effects of miR-101 and COX-2 in glioma cell proliferation and invasion was verified by CCK-8 test and Transwell assays, respectively. The present study demonstrated that miR‑101 expression was downregulated while COX‑2 was upregulated in glioma tissues and cells. Furthermore, transfection of miR‑101 significantly downregulated COX‑2 expression in both U373 and U87 glioma cells. In addition, further experiments revealed that overexpression of miR‑101 resulted in significant inhibition of the in vitro proliferation and migration of glioma cells, and the in vivo growth of established tumors. Direct downregulation of COX‑2 by transfection with corresponding small interfering RNA also inhibited the proliferation and invasion of glioma cells. These results indicate that downregulation of miR‑101 is involved in the initiation and development of glioma via COX‑2 upregulation.
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October-2016
Volume 12 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Ma C, Zheng C, Bai E and Yang K: miR‑101 inhibits glioma cell invasion via the downregulation of COX‑2. Oncol Lett 12: 2538-2544, 2016
APA
Ma, C., Zheng, C., Bai, E., & Yang, K. (2016). miR‑101 inhibits glioma cell invasion via the downregulation of COX‑2. Oncology Letters, 12, 2538-2544. https://doi.org/10.3892/ol.2016.4939
MLA
Ma, C., Zheng, C., Bai, E., Yang, K."miR‑101 inhibits glioma cell invasion via the downregulation of COX‑2". Oncology Letters 12.4 (2016): 2538-2544.
Chicago
Ma, C., Zheng, C., Bai, E., Yang, K."miR‑101 inhibits glioma cell invasion via the downregulation of COX‑2". Oncology Letters 12, no. 4 (2016): 2538-2544. https://doi.org/10.3892/ol.2016.4939