SPAG9 is overexpressed in osteosarcoma, and regulates cell proliferation and invasion through regulation of JunD

  • Authors:
    • Chi Xiao
    • Lin Fu
    • Chongnan Yan
    • Fenyong Shou
    • Qi Liu
    • Lei Li
    • Shaoqian Cui
    • Jingzhu Duan
    • Guoxin Jin
    • Jianhua Chen
    • Yuanming Bian
    • Xu Wang
    • Huan Wang
  • View Affiliations

  • Published online on: July 29, 2016     https://doi.org/10.3892/ol.2016.4920
  • Pages: 2674-2679
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Sperm-associated antigen 9 (SPAG9) is a recently characterized oncoprotein that is considered to be involved in several forms of malignant tumor. However, its biological function and expression pattern in human osteosarcoma have not yet been elucidated. In the present study, SPAG9 expression was analyzed in 58 cases of human osteosarcoma by immunohistochemistry. The results demonstrated that SPAG9 was overexpressed in 63.8% (37/58) of osteosarcoma tissues, while normal bone tissues exhibited negative SPAG9 expression. SPAG9 small interfering RNA was employed in the U2OS cell line, which has high endogenous expression, and SPAG9 transfection was performed in the MG63 cell line, which has low endogenous expression. MTT and Matrigel invasion assays demonstrated that SPAG‑9‑knockdown significantly reduced U2OS cell invasion and proliferation, while SPAG9 transfection enhanced MG63 cell proliferation and invasion. Furthermore, it was observed that SPAG9 positively regulated cyclin D1, phosphorylated‑c‑Jun NH2‑terminal kinase (JNK) and JunD expression. Treatment with the JNK inhibitor, SP600125, abolished the upregulatory effect of SPAG9 on JunD. Taken together, the present study identified SPAG9 as a critical oncoprotein involved in osteosarcoma proliferation and invasion, possibly functioning through JNK-JunD signaling.
View Figures
View References

Related Articles

Journal Cover

October-2016
Volume 12 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Xiao C, Fu L, Yan C, Shou F, Liu Q, Li L, Cui S, Duan J, Jin G, Chen J, Chen J, et al: SPAG9 is overexpressed in osteosarcoma, and regulates cell proliferation and invasion through regulation of JunD. Oncol Lett 12: 2674-2679, 2016
APA
Xiao, C., Fu, L., Yan, C., Shou, F., Liu, Q., Li, L. ... Wang, H. (2016). SPAG9 is overexpressed in osteosarcoma, and regulates cell proliferation and invasion through regulation of JunD. Oncology Letters, 12, 2674-2679. https://doi.org/10.3892/ol.2016.4920
MLA
Xiao, C., Fu, L., Yan, C., Shou, F., Liu, Q., Li, L., Cui, S., Duan, J., Jin, G., Chen, J., Bian, Y., Wang, X., Wang, H."SPAG9 is overexpressed in osteosarcoma, and regulates cell proliferation and invasion through regulation of JunD". Oncology Letters 12.4 (2016): 2674-2679.
Chicago
Xiao, C., Fu, L., Yan, C., Shou, F., Liu, Q., Li, L., Cui, S., Duan, J., Jin, G., Chen, J., Bian, Y., Wang, X., Wang, H."SPAG9 is overexpressed in osteosarcoma, and regulates cell proliferation and invasion through regulation of JunD". Oncology Letters 12, no. 4 (2016): 2674-2679. https://doi.org/10.3892/ol.2016.4920