Expression of early growth response gene‑1 in precancerous lesions of gastric cancer

  • Authors:
    • Seon‑Young Park
    • Ji‑Young Kim
    • Su‑Mi Lee
    • Jin Ook Chung
    • Kyung‑Hwa Lee
    • Chung‑Hwan Jun
    • Chang‑Hwan Park
    • Hyun‑Soo Kim
    • Sung‑Kyu Choi
    • Jong‑Sun Rew
    • Young‑Do Jung
    • Yong Han Lee
  • View Affiliations

  • Published online on: August 5, 2016     https://doi.org/10.3892/ol.2016.4962
  • Pages: 2710-2715
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Abstract

Several studies have demonstrated a correlation between the expression of early growth response gene‑1 (EGR‑1) and the progression of gastric cancers at advanced stages. However, the effects of EGR‑1 expression on human gastric cancer progression, particularly on precancerous lesions, have not been investigated. In this study, we evaluate EGR‑1 expression levels in target mucosa from patients with early gastric cancer and precancerous lesions, and assess whether EGR‑1 expression affects the oncogenic phenotypes of human gastric cancer cells. EGR‑1 protein levels were measured in tissues from subjects with normal mucosa (n=6), low‑grade dysplasia (n=6), high‑grade dysplasia (n=4) and adenocarcinoma (n=3) using enzyme‑linked immunosorbent assay and immunohistochemistry analyses. We also investigated the role of EGR‑1 in tumor cell behavior by transiently expressing a dominant active EGR‑1 variant in cultured cells. A positive correlation was observed between EGR‑1 expression and gastric carcinogenesis (P=0.016). Furthermore, there was an increase in nuclear and cytoplasmic expression of EGR‑1 in accordance with the histological grade (P for trends=0.003 and 0.003, respectively), and a positive association between the sum of the nuclear and cytoplasmic EGR‑1 expression values and the histological grade (P=0.003). In addition, transient over­expression of EGR‑1 enhanced cell proliferation, stimulated cell migration, and promoted the phosphorylation of p38 MAPK and AKT in gastric cancer cells in vitro. Our findings demonstrate that EGR‑1 may contribute to the early stages of gastric carcinogenesis via the alteration of tumor cell behaviors.
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October-2016
Volume 12 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Park SY, Kim JY, Lee SM, Chung JO, Lee KH, Jun CH, Park CH, Kim HS, Choi SK, Rew JS, Rew JS, et al: Expression of early growth response gene‑1 in precancerous lesions of gastric cancer. Oncol Lett 12: 2710-2715, 2016
APA
Park, S., Kim, J., Lee, S., Chung, J.O., Lee, K., Jun, C. ... Lee, Y.H. (2016). Expression of early growth response gene‑1 in precancerous lesions of gastric cancer. Oncology Letters, 12, 2710-2715. https://doi.org/10.3892/ol.2016.4962
MLA
Park, S., Kim, J., Lee, S., Chung, J. O., Lee, K., Jun, C., Park, C., Kim, H., Choi, S., Rew, J., Jung, Y., Lee, Y. H."Expression of early growth response gene‑1 in precancerous lesions of gastric cancer". Oncology Letters 12.4 (2016): 2710-2715.
Chicago
Park, S., Kim, J., Lee, S., Chung, J. O., Lee, K., Jun, C., Park, C., Kim, H., Choi, S., Rew, J., Jung, Y., Lee, Y. H."Expression of early growth response gene‑1 in precancerous lesions of gastric cancer". Oncology Letters 12, no. 4 (2016): 2710-2715. https://doi.org/10.3892/ol.2016.4962