Downregulated microRNA-26a modulates prostate cancer cell proliferation and apoptosis by targeting COX-2

  • Authors:
    • Jing Zhang
    • Jinghao Liang
    • Jianguo Huang
  • View Affiliations

  • Published online on: August 31, 2016     https://doi.org/10.3892/ol.2016.5070
  • Pages: 3397-3402
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Abstract

MicroRNA-26a (miR-26a) is expressed at lower levels in prostate cancer cells compared with normal prostate cells. However, the regulatory mechanism of miR‑26a in tumorigenesis and metastasis is not clear. In the present study, the expression profile of cellular miR‑26a was analyzed by reverse transcription‑quantitative polymerase chain reaction. The potential target of miR‑26a was identified by luciferase assay and western blotting. Examination of miR‑26a function was performed by transfection with miR‑26a mimics and inhibitor. It was found that miR‑26a expression was decreased in prostate cancer tissues and cell lines, with androgen‑independent prostate cancer (AIPC) showing lower miR‑26a expression compared with androgen‑dependent prostate cancer (ADPC). Overexpression of miR‑26a by transfecting miR‑26a mimics could significantly enhance apoptosis, and this upregulation of apoptosis was triggered by cytochrome c oxidase subunit II inhibition. Furthermore, it was found that lower miR‑26a density resulted in an evidently poor prognosis. Understanding the important roles of miR‑26a in regulating cell apoptosis in human prostate cancer cells may aid the exploration of AIPC transformation mechanisms and contribute to the development of miRNA-based therapy in the future.
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November-2016
Volume 12 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang J, Liang J and Huang J: Downregulated microRNA-26a modulates prostate cancer cell proliferation and apoptosis by targeting COX-2. Oncol Lett 12: 3397-3402, 2016
APA
Zhang, J., Liang, J., & Huang, J. (2016). Downregulated microRNA-26a modulates prostate cancer cell proliferation and apoptosis by targeting COX-2. Oncology Letters, 12, 3397-3402. https://doi.org/10.3892/ol.2016.5070
MLA
Zhang, J., Liang, J., Huang, J."Downregulated microRNA-26a modulates prostate cancer cell proliferation and apoptosis by targeting COX-2". Oncology Letters 12.5 (2016): 3397-3402.
Chicago
Zhang, J., Liang, J., Huang, J."Downregulated microRNA-26a modulates prostate cancer cell proliferation and apoptosis by targeting COX-2". Oncology Letters 12, no. 5 (2016): 3397-3402. https://doi.org/10.3892/ol.2016.5070