Plumbagin inhibits cell proliferation and promotes apoptosis in multiple myeloma cells through inhibition of the PI3K/Akt-mTOR pathway

  • Authors:
    • Hongwei Wu
    • Xiaozhen Dai
    • Enren Wang
  • View Affiliations

  • Published online on: August 26, 2016     https://doi.org/10.3892/ol.2016.5048
  • Pages: 3614-3618
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Abstract

Plumbagin is the primary component of the traditional Chinese medicine Baihua Dan, and possesses anti-infection and anticancer effects with the ability to enhance the sensitivity of tumor cells to radiation therapy. The present study aimed to investigate the potential anticancer effect and mechanism of plumbagin on multiple myeloma (MM) cells. Human MM OPM1 cells were treated with plumbagin, and its impact on cell viability, cytotoxicity, apoptosis and caspase‑3 activity was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, lactate dehydrogenase leakage, flow cytometry and colorimetric assays. In addition, the protein expression levels of phosphoinositide 3‑kinase, phosphorylated (p)‑Akt and p‑mammalian target of rapamycin (mTOR) in OPM1 cells were analyzed by western blotting. The results demonstrated that plumbagin treatment inhibited cell viability, increased cell cytotoxicity, activated cell apoptosis and promoted caspase‑3 activity in the OPM1 cells. Furthermore, pretreatment of plumbagin significantly suppressed PI3K, p‑Akt and p‑mTOR protein expression levels in the OPM1 cells. In conclusion, the present study indicates that plumbagin inhibits cell proliferation and promotes apoptosis in MM cells through inhibition of PI3K/Akt-mTOR expression.
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November-2016
Volume 12 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Wu H, Dai X and Wang E: Plumbagin inhibits cell proliferation and promotes apoptosis in multiple myeloma cells through inhibition of the PI3K/Akt-mTOR pathway. Oncol Lett 12: 3614-3618, 2016
APA
Wu, H., Dai, X., & Wang, E. (2016). Plumbagin inhibits cell proliferation and promotes apoptosis in multiple myeloma cells through inhibition of the PI3K/Akt-mTOR pathway. Oncology Letters, 12, 3614-3618. https://doi.org/10.3892/ol.2016.5048
MLA
Wu, H., Dai, X., Wang, E."Plumbagin inhibits cell proliferation and promotes apoptosis in multiple myeloma cells through inhibition of the PI3K/Akt-mTOR pathway". Oncology Letters 12.5 (2016): 3614-3618.
Chicago
Wu, H., Dai, X., Wang, E."Plumbagin inhibits cell proliferation and promotes apoptosis in multiple myeloma cells through inhibition of the PI3K/Akt-mTOR pathway". Oncology Letters 12, no. 5 (2016): 3614-3618. https://doi.org/10.3892/ol.2016.5048