Open Access

Effect of surgical wound fluids after intraoperative electron radiotherapy on the cancer stem cell phenotype in a panel of human breast cancer cell lines

  • Authors:
    • Karolina Zaleska
    • Wiktoria Maria Suchorska
    • Anna Przybyła
    • Dawid Murawa
  • View Affiliations

  • Published online on: September 21, 2016     https://doi.org/10.3892/ol.2016.5167
  • Pages: 3707-3714
  • Copyright: © Zaleska et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The wound healing process after surgery alters the area surrounding the original tumor and around the scar, and the modified microenvironment is more favorable for tumor recurrence. Intraoperative radiotherapy (IORT) is one of the more novel strategies in breast cancer (BC) treatment. Irradiation during surgery has effects on the tumor microenvironment, abrogating the proliferative cascade induced by surgical wound healing. The aim of the present study was to determine the effect of surgical wound fluids from IOERT treatment (RT‑WF) compared with wound fluids from conservative‑breast surgery only (WF) on the cancer stem cell phenotype in a panel of BC cell lines. Post‑operative wound fluids were derived from patients with BC who underwent a tumor resection (quadrantectomy) plus intraoperative electron radiotherapy using a single dose of ≤10 Gy on the tumor bed and surrounding tissues, or from those who underwent a tumor resection without IOERT. Cell lines were incubated with 10% wound fluids, and after 4 days, the cluster of differentiation (CD)44+/CD24‑/low phenotype and aldehyde dehydrogenase 1 (ALDH1) activity were determined by flow cytometry. The two types of fluid each affected the CD44+/CD24‑/low phenotype. The results varied markedly between each cell line, even for the same histological subtypes. RT‑WF decreased the CD44+/CD24‑/low populations in the basal‑like BT‑549 and MDA‑MB‑468 cell lines, whereas in the luminal type MCF7 cell line, the two fluids inhibited these populations. The HER‑OE subtypes harbored a minimal CD44+/CD24‑/low population, but the growth of SK‑BR‑3 was stimulated by the two post-operative fluids. WF exhibited a stronger effect on ALDH1 activity compared with RT‑WF. The stimulatory effect was dependent on the histological subtype of the cell line and the strongest dependence was observed in luminal subtypes characterized by low dehydrogenase activity in the control group. The present results enable a better understanding of the mechanism of recurrence and metastases following BC surgery. With respect to histological phenotype, its effect on tumor progression, either local or systemic, strongly suggests the requirement for further research and clinical validation.
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November-2016
Volume 12 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zaleska K, Suchorska WM, Przybyła A and Murawa D: Effect of surgical wound fluids after intraoperative electron radiotherapy on the cancer stem cell phenotype in a panel of human breast cancer cell lines. Oncol Lett 12: 3707-3714, 2016
APA
Zaleska, K., Suchorska, W.M., Przybyła, A., & Murawa, D. (2016). Effect of surgical wound fluids after intraoperative electron radiotherapy on the cancer stem cell phenotype in a panel of human breast cancer cell lines. Oncology Letters, 12, 3707-3714. https://doi.org/10.3892/ol.2016.5167
MLA
Zaleska, K., Suchorska, W. M., Przybyła, A., Murawa, D."Effect of surgical wound fluids after intraoperative electron radiotherapy on the cancer stem cell phenotype in a panel of human breast cancer cell lines". Oncology Letters 12.5 (2016): 3707-3714.
Chicago
Zaleska, K., Suchorska, W. M., Przybyła, A., Murawa, D."Effect of surgical wound fluids after intraoperative electron radiotherapy on the cancer stem cell phenotype in a panel of human breast cancer cell lines". Oncology Letters 12, no. 5 (2016): 3707-3714. https://doi.org/10.3892/ol.2016.5167