Inhibitory effects of Chinese nutritional herbs in isogenic breast carcinoma cells with modulated estrogen receptor function

  • Authors:
    • Nitin Telang
    • Guo Li
    • Meena Katdare
    • Daniel Sepkovic
    • Leon Bradlow
    • George Wong
  • View Affiliations

  • Published online on: September 28, 2016     https://doi.org/10.3892/ol.2016.5197
  • Pages: 3949-3957
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Abstract

In estrogen receptor (ER)+ MCF‑7 cells, ER represents a ligand-activated transcription factor, and 17β-estradiol (E2) represents its physiological ligand. Maintenance of the human breast carcinoma‑derived MCF‑7 cells with 0.7% serum selected a proliferative sub‑population of E2‑responsive cells with transiently non‑functional ER due to limited availability of E2. Culture of MCF‑7 cells in the presence of either 0.7% serum, <1 nM E2 or 0.7% serum + 20 nM E2 selected isogenic cells with either non‑functional ER (ER‑NF) or functional ER (ER‑F) phenotype. The two phenotypes responded to the growth‑promoting effects of E2 and to the growth‑inhibitory effects of the selective ER modulator tamoxifen, indicating retention of E2 responsiveness. Comparative dose‑response experiments with Chinese nutritional herbs on ER‑NF and ER‑F cells identified the inhibitory concentration (IC)50 values for these herbs, while the IC50 ratios for the ER‑NF:ER‑F phenotypes facilitated their rank ordering in terms of efficacy. Out of the 11 efficacious herbs tested, five herbs exhibited ER‑F > ER‑NF inhibitory activity, four exhibited ER‑F = ER‑NF inhibitory activity and two exhibited ER‑NF > ER‑F inhibitory activity. Extracts from representative herbs, Lycium barbarum bark, Epimedium grandiflorum and Cornus officinalis, from each of the three groups inhibited anchorage‑independent growth, induced G1 or G2/M arrest and/or apoptosis, and generated anti‑proliferative E2 metabolites. The differential growth inhibition in ER‑NF and ER‑F phenotypes, together with the mechanistic efficacy of representative herbs, identified potential leads for their efficacy on ER+ and/or ER‑ breast cancer.
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November-2016
Volume 12 Issue 5

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Online ISSN:1792-1082

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Spandidos Publications style
Telang N, Li G, Katdare M, Sepkovic D, Bradlow L and Wong G: Inhibitory effects of Chinese nutritional herbs in isogenic breast carcinoma cells with modulated estrogen receptor function. Oncol Lett 12: 3949-3957, 2016
APA
Telang, N., Li, G., Katdare, M., Sepkovic, D., Bradlow, L., & Wong, G. (2016). Inhibitory effects of Chinese nutritional herbs in isogenic breast carcinoma cells with modulated estrogen receptor function. Oncology Letters, 12, 3949-3957. https://doi.org/10.3892/ol.2016.5197
MLA
Telang, N., Li, G., Katdare, M., Sepkovic, D., Bradlow, L., Wong, G."Inhibitory effects of Chinese nutritional herbs in isogenic breast carcinoma cells with modulated estrogen receptor function". Oncology Letters 12.5 (2016): 3949-3957.
Chicago
Telang, N., Li, G., Katdare, M., Sepkovic, D., Bradlow, L., Wong, G."Inhibitory effects of Chinese nutritional herbs in isogenic breast carcinoma cells with modulated estrogen receptor function". Oncology Letters 12, no. 5 (2016): 3949-3957. https://doi.org/10.3892/ol.2016.5197