MicroRNA-181b is downregulated in non-small cell lung cancer and inhibits cell motility by directly targeting HMGB1

  • Authors:
    • Yun Liu
    • Xu Hu
    • Daokui Xia
    • Songlin Zhang
  • View Affiliations

  • Published online on: September 28, 2016     https://doi.org/10.3892/ol.2016.5198
  • Pages: 4181-4186
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Abstract

The expression of microRNA-181b (miR-181b) has been investigated in various human cancers. However, the expression and functions of miR-181b in non‑small cell lung cancer (NSCLC) are yet to be studied. In the present study, miR‑181b expression in NSCLC tissues and cell lines was analyzed by quantitative polymerase chain reaction, and was shown to be recurrently downregulated. Following transfection of the H23 and H522 NSCLC cells lines with miR‑181b, cell migration and cell invasion assays were performed to evaluate the effect of miR‑181b overexpression on the cell motility. It was demonstrated that overexpression of miR‑181b inhibited the migration and invasion of NSCLC cells. Subsequently, bioinformatics analysis, western blotting and luciferase reporter assays were conducted to investigate the mechanism underlying the miR‑181b‑mediated inhibition of NSCLC cell motility. It was found that miR‑181b directly targeted high‑mobility group box‑1 (HMGB1) in NSCLC cells. These results reveal a novel therapeutic target, the miR‑181b/HMGB1 axis, in NSCLC. Treatment approaches targeting this axis will be beneficial to prevent NSCLC from becoming invasive.
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November-2016
Volume 12 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Liu Y, Hu X, Xia D and Zhang S: MicroRNA-181b is downregulated in non-small cell lung cancer and inhibits cell motility by directly targeting HMGB1. Oncol Lett 12: 4181-4186, 2016
APA
Liu, Y., Hu, X., Xia, D., & Zhang, S. (2016). MicroRNA-181b is downregulated in non-small cell lung cancer and inhibits cell motility by directly targeting HMGB1. Oncology Letters, 12, 4181-4186. https://doi.org/10.3892/ol.2016.5198
MLA
Liu, Y., Hu, X., Xia, D., Zhang, S."MicroRNA-181b is downregulated in non-small cell lung cancer and inhibits cell motility by directly targeting HMGB1". Oncology Letters 12.5 (2016): 4181-4186.
Chicago
Liu, Y., Hu, X., Xia, D., Zhang, S."MicroRNA-181b is downregulated in non-small cell lung cancer and inhibits cell motility by directly targeting HMGB1". Oncology Letters 12, no. 5 (2016): 4181-4186. https://doi.org/10.3892/ol.2016.5198