Open Access

Protective effect of selenium against cisplatin-induced nasopharyngeal cancer in male albino rats

  • Authors:
    • Kan Zhu
    • Lei Jiang
    • Yun Chu
    • Yong‑Sheng Zhang
  • View Affiliations

  • Published online on: November 2, 2016     https://doi.org/10.3892/ol.2016.5346
  • Pages: 5068-5074
  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study evaluated the protective effect of selenium against cisplatin-induced nasopharyngeal cancer in the cardiac tissue of adult rats. Male Wistar rats were divided into 4 groups of 6 rats each. Different combinations of selenium and cisplatin were administered for 45 days, following which the animals were sacrificed and the nasopharyngeal tissue was surgically removed. Lipid peroxidation, reduced glutathione, and the antioxidant enzymes superoxide dismutase (SOD), catalase and lactate dehydrogenase (LDH) were measured. Apoptotic‑related gene (p53, bax and caspase 3) mRNA expression was determined using quantitative polymerase chain reaction. Caspase 3 activity was also measured in all the groups. The results demonstrated that selenium significantly reduced the levels of malondialdehyde. The levels of glutathione, SOD, LDH and catalase significantly increased following selenium treatment. Relative mRNA expression (p53, bax and caspase 3) was significantly reduced in the cisplatin-treated rats, but it significantly increased following selenium treatment. The anticancer activity of selenium was also investigated in HK1cells. Fluorescence and confocal microscopy were used to analyze apoptosis and reactive oxygen species. The protective effect of selenium was also evident through caspase 3 activity, which significantly increased following selenium treatment. Taken together, these results indicate that selenium may be beneficial against cisplatin-induced nasopharyngeal cancer.
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December-2016
Volume 12 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhu K, Jiang L, Chu Y and Zhang YS: Protective effect of selenium against cisplatin-induced nasopharyngeal cancer in male albino rats. Oncol Lett 12: 5068-5074, 2016
APA
Zhu, K., Jiang, L., Chu, Y., & Zhang, Y. (2016). Protective effect of selenium against cisplatin-induced nasopharyngeal cancer in male albino rats. Oncology Letters, 12, 5068-5074. https://doi.org/10.3892/ol.2016.5346
MLA
Zhu, K., Jiang, L., Chu, Y., Zhang, Y."Protective effect of selenium against cisplatin-induced nasopharyngeal cancer in male albino rats". Oncology Letters 12.6 (2016): 5068-5074.
Chicago
Zhu, K., Jiang, L., Chu, Y., Zhang, Y."Protective effect of selenium against cisplatin-induced nasopharyngeal cancer in male albino rats". Oncology Letters 12, no. 6 (2016): 5068-5074. https://doi.org/10.3892/ol.2016.5346