Open Access

Identification of proteins associated with pediatric bilateral Wilms tumor

  • Authors:
    • Zechen Yan
    • Qingjun Meng
    • Jinjian Yang
    • Junjie Zhang
    • Wei Zhao
    • Fei Guo
    • Dongjian Song
    • Yuxiao Zhan
    • Dandan Fan
    • Ruiyi Zhou
    • Shuqiang Zuo
    • Zehua Wang
    • Jiekai Yu
    • Shu Zheng
    • Jiaxiang Wang
  • View Affiliations

  • Published online on: October 21, 2016     https://doi.org/10.3892/ol.2016.5306
  • Pages: 5075-5079
  • Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Wilms tumor (WT) is the most common cancer that primarily develops in abdominal solid organ of children. It has no incipient symptom, and the most frequent symptoms are a painless, palpable abdominal mass. Proteomics technology was used to select the differentially expressed proteins of bilateral Wilms tumor (BWT). Ten serum samples of children with BWT were chosen, 20 serum samples of children with unilateral WT (UWT) and 20 serum samples of healthy children were selected, and proteomics technology was used to detect and collect data. Using bioinformatics, the data were analyzed and 10 difference peaks were obtained (P<0.01). Non‑linear support vector machine was used to classify and to select the composite pattern with the highest Youden's index, and one differentially expressed protein with m/z of 5,648 kDa was obtained. A significantly high expression in children with BWT was obtained, and the expression intensity was also significantly (3,889.36±1,796.83) higher for children with BWT compared to those with UWT (2,886.81±1,404.65) and healthy children (432.21±730.42). Matrix‑assisted laser desorption ionization/time‑of‑flight ionization/time‑of‑flight mass spectrometry was used for identification of the peak, and the peak was further identified as apolipoprotein C‑Ⅲ (APO C‑Ⅲ) by western blot analysis. In conclusion, to the best of our knowledge, a differentially expressed protein of APO C‑Ⅲ of BWT was obtained through proteomics technology for the first time, and it is expected to be a new marker for the early diagnosis and prognosis of BWT.
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December-2016
Volume 12 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yan Z, Meng Q, Yang J, Zhang J, Zhao W, Guo F, Song D, Zhan Y, Fan D, Zhou R, Zhou R, et al: Identification of proteins associated with pediatric bilateral Wilms tumor. Oncol Lett 12: 5075-5079, 2016
APA
Yan, Z., Meng, Q., Yang, J., Zhang, J., Zhao, W., Guo, F. ... Wang, J. (2016). Identification of proteins associated with pediatric bilateral Wilms tumor. Oncology Letters, 12, 5075-5079. https://doi.org/10.3892/ol.2016.5306
MLA
Yan, Z., Meng, Q., Yang, J., Zhang, J., Zhao, W., Guo, F., Song, D., Zhan, Y., Fan, D., Zhou, R., Zuo, S., Wang, Z., Yu, J., Zheng, S., Wang, J."Identification of proteins associated with pediatric bilateral Wilms tumor". Oncology Letters 12.6 (2016): 5075-5079.
Chicago
Yan, Z., Meng, Q., Yang, J., Zhang, J., Zhao, W., Guo, F., Song, D., Zhan, Y., Fan, D., Zhou, R., Zuo, S., Wang, Z., Yu, J., Zheng, S., Wang, J."Identification of proteins associated with pediatric bilateral Wilms tumor". Oncology Letters 12, no. 6 (2016): 5075-5079. https://doi.org/10.3892/ol.2016.5306