KIAA1377 is associated with lymph node metastasis in esophageal squamous cell carcinoma

  • Authors:
    • Shu‑Tao Zheng
    • Chen‑Chen Yang
    • Qing Liu
    • Tao Liu
    • Mang Lu
    • Fang Dai
    • Xiang‑Peng Gao
    • Ilyar Sheyhidin
    • Xiao‑Mei Lu
  • View Affiliations

  • Published online on: November 2, 2016     https://doi.org/10.3892/ol.2016.5343
  • Pages: 5223-5228
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Abstract

KIAA1377, of which there are few studies regarding cell biology and neurological diseases, has been found to be significantly amplified in esophageal squamous cell carcinoma (ESCC) with lymph node metastasis compared with ESCC without lymph node metastasis. This suggests that KIAA1377 may play a role in the lymph node metastasis of ESCC. There has, to the best of our knowledge, been no study performed to investigate the role of KIAA1377 in ESCC. In the present study, the expression of KIAA1377 was detected by immunohistochemistry, and its expression was statistically analyzed with clinicopathological parameters, using commercially obtained tissue arrays consisting of 86 cases of ESCC and 79 paired controls. KIAA1377 was knocked down ex vivo using transient transfection with specific small hairpin RNA (shRNA) vectors into ESCC TE‑1 and EC9706 cell lines whose endogenous KIAA1377 level was highest. The variation of proliferation, migration and invasion were evaluated using methyl thiazolyl tetrazolium, wound healing and Transwell assay, respectively. It was found in vivo that KIAA1377 expression was significantly associated with lymph node metastasis and differentiation, and ex vivo that knockdown of KIAA1377 cannot significantly affect proliferation and mobility in the ESCC cell line TE‑1. Overall, this is the first study suggesting that KIAA1377 may play a role in the lymph node micrometastasis of ESCC.
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December-2016
Volume 12 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zheng ST, Yang CC, Liu Q, Liu T, Lu M, Dai F, Gao XP, Sheyhidin I and Lu XM: KIAA1377 is associated with lymph node metastasis in esophageal squamous cell carcinoma. Oncol Lett 12: 5223-5228, 2016
APA
Zheng, S., Yang, C., Liu, Q., Liu, T., Lu, M., Dai, F. ... Lu, X. (2016). KIAA1377 is associated with lymph node metastasis in esophageal squamous cell carcinoma. Oncology Letters, 12, 5223-5228. https://doi.org/10.3892/ol.2016.5343
MLA
Zheng, S., Yang, C., Liu, Q., Liu, T., Lu, M., Dai, F., Gao, X., Sheyhidin, I., Lu, X."KIAA1377 is associated with lymph node metastasis in esophageal squamous cell carcinoma". Oncology Letters 12.6 (2016): 5223-5228.
Chicago
Zheng, S., Yang, C., Liu, Q., Liu, T., Lu, M., Dai, F., Gao, X., Sheyhidin, I., Lu, X."KIAA1377 is associated with lymph node metastasis in esophageal squamous cell carcinoma". Oncology Letters 12, no. 6 (2016): 5223-5228. https://doi.org/10.3892/ol.2016.5343