Interaction of cyclophilin A with a novel binding protein, SR-25, and characterization of their expression pattern in Chinese hepatocellular carcinoma patients

  • Authors:
    • Jian Chen
    • Ning Li
    • Peiwen Lian
    • Jiahui Wang
    • Peng Li
    • Zhaohua Gong
    • Lixin Jiang
  • View Affiliations

  • Published online on: November 7, 2016     https://doi.org/10.3892/ol.2016.5357
  • Pages: 5254-5260
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Abstract

Cyclophilin (Cyp) A has been reported to be overexpressed in the majority of cancer cells, including hepatocellular carcinoma (HCC). However, the biological functions of CypA in HCC are far from being understood. To determine the biological functions of CypA in HCC, the present study screened human fetal liver complementary DNA for proteins interacting with CypA using the yeast two‑hybrid system. A nuclear protein, serine/arginine-rich (SR)‑25, was isolated as a novel CypA-binding protein that is distinct from those previously described in the literature. Binding assays and co‑immunoprecipitation confirmed the physical association between CypA and SR‑25. The present study demonstrated that CypA may interact with SR‑25 through its peptidyl‑prolyl isomerase domain. In addition, CypA may induce the expression of SR‑25 in Hep3B cells. The messenger RNA levels of CypA and SR‑25 in HCC indicated that there was a significant correlation between the expression of CypA and the expression of SR‑25 in HCC. It can be speculated that the interaction between CypA and SR‑25 proteins may be involved in potential carcinogenic functions of CypA in HCC. Further studies will focus on elucidating in detail the molecular mechanisms of the interaction between CypA and SR-25.
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December-2016
Volume 12 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Chen J, Li N, Lian P, Wang J, Li P, Gong Z and Jiang L: Interaction of cyclophilin A with a novel binding protein, SR-25, and characterization of their expression pattern in Chinese hepatocellular carcinoma patients. Oncol Lett 12: 5254-5260, 2016
APA
Chen, J., Li, N., Lian, P., Wang, J., Li, P., Gong, Z., & Jiang, L. (2016). Interaction of cyclophilin A with a novel binding protein, SR-25, and characterization of their expression pattern in Chinese hepatocellular carcinoma patients. Oncology Letters, 12, 5254-5260. https://doi.org/10.3892/ol.2016.5357
MLA
Chen, J., Li, N., Lian, P., Wang, J., Li, P., Gong, Z., Jiang, L."Interaction of cyclophilin A with a novel binding protein, SR-25, and characterization of their expression pattern in Chinese hepatocellular carcinoma patients". Oncology Letters 12.6 (2016): 5254-5260.
Chicago
Chen, J., Li, N., Lian, P., Wang, J., Li, P., Gong, Z., Jiang, L."Interaction of cyclophilin A with a novel binding protein, SR-25, and characterization of their expression pattern in Chinese hepatocellular carcinoma patients". Oncology Letters 12, no. 6 (2016): 5254-5260. https://doi.org/10.3892/ol.2016.5357