Open Access

Longikaurin A, a natural ent-kaurane, suppresses stemness in nasopharyngeal carcinoma cells

  • Authors:
    • Yan Yuan
    • Yong Du
    • Xiao‑Ye Hu
    • Mei‑Yuan Liu
    • Ji‑Ke Du
    • Xue‑Min Liu
    • Hong‑En Yu
    • Tian‑Zhu Wang
    • Jian‑Xin Pu
    • Qian Zhong
    • Qing‑Feng Zou
  • View Affiliations

  • Published online on: January 19, 2017     https://doi.org/10.3892/ol.2017.5625
  • Pages: 1672-1680
  • Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cancer stem cells (CSCs) are a small proportion of tumor cells that may be responsible for tumor metastasis and recurrence. Our recent research indicated that longikaurin A (LK‑A) exhibited anti‑tumor activity in nasopharyngeal carcinoma (NPC) both in vitro and in vivo. Here, we further investigated whether LK‑A could suppress the stemness of NPC cells. Sphere formation assay was used to assess the self‑renewal ability of the cells treated with LK‑A. Side population (SP) was determined by flow cytometry to measure the influence of LK‑A on NPC SPs. The expression of the c‑myc and fibronectin was detected by western blotting. The cytotoxicity of LK‑A in combination with cisplatin to NPC cells was determined by MTT assay. Colony formation assay was used to verify whether LK‑A could sensitize NPC cells to radiation and reverse the radiotherapy resistance. In the present study, we found that LK‑A reduced the number and size of spheroid formation and decreased the SP cell percentage of the S18 cell line at a low concentration. Furthermore, LK‑A treatment downregulated the expression of c‑myc and fibronectin in NPC cell lines. Moreover, LK‑A could significantly enhance the chemotherapeutic and radiotherapeutic sensitivity of NPC cell lines and reverse acquired radiotherapy resistance of Sune2‑IR. Our data revealed that LK‑A could suppress the stemness of NPC cells and may enhance the efficacy of radiotherapy and chemotherapy.
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March-2017
Volume 13 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Yuan Y, Du Y, Hu XY, Liu MY, Du JK, Liu XM, Yu HE, Wang TZ, Pu JX, Zhong Q, Zhong Q, et al: Longikaurin A, a natural ent-kaurane, suppresses stemness in nasopharyngeal carcinoma cells. Oncol Lett 13: 1672-1680, 2017
APA
Yuan, Y., Du, Y., Hu, X., Liu, M., Du, J., Liu, X. ... Zou, Q. (2017). Longikaurin A, a natural ent-kaurane, suppresses stemness in nasopharyngeal carcinoma cells. Oncology Letters, 13, 1672-1680. https://doi.org/10.3892/ol.2017.5625
MLA
Yuan, Y., Du, Y., Hu, X., Liu, M., Du, J., Liu, X., Yu, H., Wang, T., Pu, J., Zhong, Q., Zou, Q."Longikaurin A, a natural ent-kaurane, suppresses stemness in nasopharyngeal carcinoma cells". Oncology Letters 13.3 (2017): 1672-1680.
Chicago
Yuan, Y., Du, Y., Hu, X., Liu, M., Du, J., Liu, X., Yu, H., Wang, T., Pu, J., Zhong, Q., Zou, Q."Longikaurin A, a natural ent-kaurane, suppresses stemness in nasopharyngeal carcinoma cells". Oncology Letters 13, no. 3 (2017): 1672-1680. https://doi.org/10.3892/ol.2017.5625