Regorafenib diminishes the expression and secretion of angiogenesis and metastasis associated proteins and inhibits cell invasion via NF-κB inactivation in SK-Hep1 cells

  • Authors:
    • Yu‑Chang Liu
    • Reng‑Hong Wu
    • Wei‑Shu Wang
  • View Affiliations

  • Published online on: May 9, 2017     https://doi.org/10.3892/ol.2017.6142
  • Pages: 461-467
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Abstract

The aim of the present study was to investigate the effects of regorafenib on the nuclear factor κ‑light‑chain‑enhancer of activated B cells (NF)‑κB‑modulated expression of angiogenesis‑ and metastasis‑associated proteins and cell invasion in human hepatocellular carcinoma SK‑Hep1 cells. The SK‑Hep1 cells were treated with different concentrations of NF‑κB inhibitor 4‑N‑[2‑(4‑phenoxyphenyl) ethyl] quinazoline‑4,6‑diamine (QNZ) or regorafenib for 24 or 48 h. The effects of QNZ and regorafenib on cell viability, NF‑κB activation, expression and secretion levels of angiogenesis‑ and metastasis‑associated proteins and cell invasion were evaluated with MTT assays, western blotting, ELISA, gelatin zymography and cell invasion assays. The results demonstrated that QNZ and regorafenib significantly reduced the expression and secretion levels of the angiogenesis‑ and metastasis‑associated proteins vascular endothelial growth factor, tumor necrosis factor‑α, interleukin (IL)‑1β, IL‑6, matrix metalloproteinase (MMP)‑2 and MMP‑9, NF‑κB activation and cell invasion. In conclusion, the inhibition of NF‑κB activation induces anti‑angiogenic and antimetastatic effects in SK‑Hep1 cells. Regorafenib reduces the level of expression and secretion of angiogenesis‑ and metastasis‑associated proteins and cell invasion through the suppression of NF‑κB activation in SK‑Hep1 cells.
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July-2017
Volume 14 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Liu YC, Wu RH and Wang WS: Regorafenib diminishes the expression and secretion of angiogenesis and metastasis associated proteins and inhibits cell invasion via NF-κB inactivation in SK-Hep1 cells. Oncol Lett 14: 461-467, 2017
APA
Liu, Y., Wu, R., & Wang, W. (2017). Regorafenib diminishes the expression and secretion of angiogenesis and metastasis associated proteins and inhibits cell invasion via NF-κB inactivation in SK-Hep1 cells. Oncology Letters, 14, 461-467. https://doi.org/10.3892/ol.2017.6142
MLA
Liu, Y., Wu, R., Wang, W."Regorafenib diminishes the expression and secretion of angiogenesis and metastasis associated proteins and inhibits cell invasion via NF-κB inactivation in SK-Hep1 cells". Oncology Letters 14.1 (2017): 461-467.
Chicago
Liu, Y., Wu, R., Wang, W."Regorafenib diminishes the expression and secretion of angiogenesis and metastasis associated proteins and inhibits cell invasion via NF-κB inactivation in SK-Hep1 cells". Oncology Letters 14, no. 1 (2017): 461-467. https://doi.org/10.3892/ol.2017.6142