Open Access

Downregulation of miR‑186 is associated with metastatic recurrence of gastrointestinal stromal tumors

  • Authors:
    • Takeshi Niinuma
    • Masahiro Kai
    • Hiroshi Kitajima
    • Eiichiro Yamamoto
    • Taku Harada
    • Reo Maruyama
    • Takayuki Nobuoka
    • Toshirou Nishida
    • Tatsuo Kanda
    • Tadashi Hasegawa
    • Takashi Tokino
    • Tamotsu Sugai
    • Yasuhisa Shinomura
    • Hiroshi Nakase
    • Hiromu Suzuki
  • View Affiliations

  • Published online on: September 8, 2017     https://doi.org/10.3892/ol.2017.6911
  • Pages: 5703-5710
  • Copyright: © Niinuma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Although dysregulation of microRNAs (miRNAs/miRs) is a common feature of human malignancies, its involvement in gastrointestinal stromal tumors (GISTs) is not fully understood. The present study aimed to identify the miRNAs that perform a role in GIST metastasis. miRNA expression profiles from a series of 32 primary GISTs were analyzed using microarrays, and miR‑186 was observed to be downregulated in tumors exhibiting metastatic recurrence. Reverse transcription‑quantitative polymerase chain reaction analysis of an independent cohort of 100 primary GISTs revealed that low miR‑186 expression is associated with metastatic recurrence and a poor prognosis. Inhibition of miR‑186 in GIST‑T1 cells promoted cell migration. Gene expression microarray analysis demonstrated that miR‑186 inhibition upregulated a set of genes implicated in cancer metastasis, including insulin‑like growth factor‑binding protein 3, AKT serine/threonine kinase 2, hepatocyte growth factor receptor, CXC chemokine receptor 4 and epidermal growth factor‑containing fibulin‑like extracellular matrix protein 1. These results suggest that the downregulation of miR‑186 is involved in the metastatic recurrence of GISTs, and that miR‑186 levels could potentially be a predictive biomarker for clinical outcome.
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November-2017
Volume 14 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Niinuma T, Kai M, Kitajima H, Yamamoto E, Harada T, Maruyama R, Nobuoka T, Nishida T, Kanda T, Hasegawa T, Hasegawa T, et al: Downregulation of miR‑186 is associated with metastatic recurrence of gastrointestinal stromal tumors. Oncol Lett 14: 5703-5710, 2017
APA
Niinuma, T., Kai, M., Kitajima, H., Yamamoto, E., Harada, T., Maruyama, R. ... Suzuki, H. (2017). Downregulation of miR‑186 is associated with metastatic recurrence of gastrointestinal stromal tumors. Oncology Letters, 14, 5703-5710. https://doi.org/10.3892/ol.2017.6911
MLA
Niinuma, T., Kai, M., Kitajima, H., Yamamoto, E., Harada, T., Maruyama, R., Nobuoka, T., Nishida, T., Kanda, T., Hasegawa, T., Tokino, T., Sugai, T., Shinomura, Y., Nakase, H., Suzuki, H."Downregulation of miR‑186 is associated with metastatic recurrence of gastrointestinal stromal tumors". Oncology Letters 14.5 (2017): 5703-5710.
Chicago
Niinuma, T., Kai, M., Kitajima, H., Yamamoto, E., Harada, T., Maruyama, R., Nobuoka, T., Nishida, T., Kanda, T., Hasegawa, T., Tokino, T., Sugai, T., Shinomura, Y., Nakase, H., Suzuki, H."Downregulation of miR‑186 is associated with metastatic recurrence of gastrointestinal stromal tumors". Oncology Letters 14, no. 5 (2017): 5703-5710. https://doi.org/10.3892/ol.2017.6911