Open Access

Fatty acid synthase affects expression of ErbB receptors in epithelial to mesenchymal transition of breast cancer cells and invasive ductal carcinoma

  • Authors:
    • Tingting Chen
    • Lan Zhou
    • Hua Li
    • Yuan Tian
    • Junqin Li
    • Lihua Dong
    • Yuhua Zhao
    • Dapeng Wei
  • View Affiliations

  • Published online on: September 15, 2017     https://doi.org/10.3892/ol.2017.6954
  • Pages: 5934-5946
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate changes in the expression of ErbBs during epithelial‑mesenchymal transition (EMT) of breast cancer cells and its association with the expression of fatty acid synthase (FASN). MCF‑7‑MEK5 cells were used as the experimental model, while MCF‑7 cells were used as a control. Tumor cells were implanted into nude mice for in vivo analysis. Cerulenin was used as a FASN inhibitor. Reverse transcription‑polymerase chain reaction and western blot analysis were used to detect expression levels of FASN and ErbB1‑4. Immunohistochemistry was used to detect the expression of FASN and ErbB1‑4 in 58 invasive ductal carcinomas (IDC), as well as their association with clinicopathological characteristics. The expression of FASN and ErbB1‑4 in MCF‑7‑MEK5 cells and tumor tissues increased significantly compared with controls (P<0.001). Inhibition of FASN by cerulenin resulted in a significant decrease in expression of ErbB1, 2 and 4 (P<0.001), whereas there was no evident change in ErbB3. In IDC samples, the expression of FASN and ErbB1‑4 increased considerably in lymph node metastases compared with non‑lymph node metastases (P<0.05). ErbB2 expression increased in advanced clinical stages (II, III and IV) of IDC and in tumors with larger diameters (P<0.05). The expression of ErbB3 increased in ER‑positive tumors (P<0.05). Additionally, a positive association between the expression of FASN and ErbB1, 2 and 4 was observed (P<0.05). FASN activates ErbB1, 2 and 4, and their dimers, which are polymerized via the microstructural domain of the cell membrane. This may initiate EMT and consequentlyincrease the invasion and migration of cancer cells. However, ErbB3 may also affect tumor progression via a FASN‑independent pathway.
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November-2017
Volume 14 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Chen T, Zhou L, Li H, Tian Y, Li J, Dong L, Zhao Y and Wei D: Fatty acid synthase affects expression of ErbB receptors in epithelial to mesenchymal transition of breast cancer cells and invasive ductal carcinoma. Oncol Lett 14: 5934-5946, 2017
APA
Chen, T., Zhou, L., Li, H., Tian, Y., Li, J., Dong, L. ... Wei, D. (2017). Fatty acid synthase affects expression of ErbB receptors in epithelial to mesenchymal transition of breast cancer cells and invasive ductal carcinoma. Oncology Letters, 14, 5934-5946. https://doi.org/10.3892/ol.2017.6954
MLA
Chen, T., Zhou, L., Li, H., Tian, Y., Li, J., Dong, L., Zhao, Y., Wei, D."Fatty acid synthase affects expression of ErbB receptors in epithelial to mesenchymal transition of breast cancer cells and invasive ductal carcinoma". Oncology Letters 14.5 (2017): 5934-5946.
Chicago
Chen, T., Zhou, L., Li, H., Tian, Y., Li, J., Dong, L., Zhao, Y., Wei, D."Fatty acid synthase affects expression of ErbB receptors in epithelial to mesenchymal transition of breast cancer cells and invasive ductal carcinoma". Oncology Letters 14, no. 5 (2017): 5934-5946. https://doi.org/10.3892/ol.2017.6954