Epigallocatechin‑3‑gallate induces apoptosis in acute promyelocytic leukemia cells via a SHP‑1‑p38α MAPK‑Bax cascade

  • Authors:
    • Liugen Gan
    • Liang Zhong
    • Zhiling Shan
    • Chunlan Xiao
    • Ting Xu
    • Hao Song
    • Liu Li
    • Rong Yang
    • Beizhong Liu
  • View Affiliations

  • Published online on: September 18, 2017     https://doi.org/10.3892/ol.2017.6980
  • Pages: 6314-6320
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Abstract

Acute promyelocytic leukemia (APL) is characterized by a specific chromosomal translation, resulting in a fusion gene that affects the differentiation, proliferation and apoptosis of APL cells. Epigallocatechin‑3‑gallate (EGCG), a catechin, exhibits numerous biological functions, including antitumor activities. Previous studies have reported that EGCG induces apoptosis in NB4 cells. However, the molecular mechanism underlying EGCG‑induced apoptosis remains unclear. The present study aimed to determine the molecular basis of EGCG‑induced apoptosis in NB4 cells. EGCG treatment significantly inhibited the viability of NB4 cells in a dose‑dependent manner. In addition, EGCG treatment induced apoptosis and increased the levels of (Bcl‑2‑like protein 4) Bax protein expression. Moreover, EGCG treatment was able to increase phosphorylated (p)‑p38α mitogen‑activated protein kinase (MAPK) and Src homology 1 domain‑containing protein tyrosine phosphatase (SHP‑1) expression. Pretreatment with PD169316 (a p38 MAPK inhibitor) partially blocked EGCG‑induced apoptosis and inhibited EGCG‑mediated Bax expression. Similarly, pretreatment with NSC87877, an inhibitor of SHP‑1, partially blocked EGCG‑induced apoptosis and inhibited EGCG‑mediated increases in p‑p38α MAPK and Bax expression. Therefore, the results of the present study indicate that EGCG is able to induce apoptosis in NB4 cells via the SHP‑1‑p38αMAPK‑Bax cascade.
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November-2017
Volume 14 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Gan L, Zhong L, Shan Z, Xiao C, Xu T, Song H, Li L, Yang R and Liu B: Epigallocatechin‑3‑gallate induces apoptosis in acute promyelocytic leukemia cells via a SHP‑1‑p38α MAPK‑Bax cascade. Oncol Lett 14: 6314-6320, 2017
APA
Gan, L., Zhong, L., Shan, Z., Xiao, C., Xu, T., Song, H. ... Liu, B. (2017). Epigallocatechin‑3‑gallate induces apoptosis in acute promyelocytic leukemia cells via a SHP‑1‑p38α MAPK‑Bax cascade. Oncology Letters, 14, 6314-6320. https://doi.org/10.3892/ol.2017.6980
MLA
Gan, L., Zhong, L., Shan, Z., Xiao, C., Xu, T., Song, H., Li, L., Yang, R., Liu, B."Epigallocatechin‑3‑gallate induces apoptosis in acute promyelocytic leukemia cells via a SHP‑1‑p38α MAPK‑Bax cascade". Oncology Letters 14.5 (2017): 6314-6320.
Chicago
Gan, L., Zhong, L., Shan, Z., Xiao, C., Xu, T., Song, H., Li, L., Yang, R., Liu, B."Epigallocatechin‑3‑gallate induces apoptosis in acute promyelocytic leukemia cells via a SHP‑1‑p38α MAPK‑Bax cascade". Oncology Letters 14, no. 5 (2017): 6314-6320. https://doi.org/10.3892/ol.2017.6980