Open Access

Expression of NF-κB and PTEN in osteosarcoma and its clinical significance

  • Authors:
    • Teng Gong
    • Xuetao Su
    • Qun Xia
    • Jinggui Wang
    • Shilian Kan
  • View Affiliations

  • Published online on: September 15, 2017     https://doi.org/10.3892/ol.2017.6972
  • Pages:6744-6748
  • Copyright: © Gong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

We investigated the role of nuclear factor-κB (NF-κB) and phosphatase and tensin homolog deleted in chromosome 10 (PTEN) in the pathogenesis of osteosarcoma and its relationship with prognosis. Immunohistochemical method was used to detect the expression of NF-κB and PTEN in osteosarcoma and adjacent tissues. RT-PCR was used to detect the expression of NF-κB and PTEN mRNA in osteosarcoma and adjacent tissues. Western blotting was used to detect the expression of NF-κB and PTEN in osteosarcoma and adjacent tissues and compare their differences. The expression of NF-κB and PTEN was detected in osteosarcoma and adjacent tissues. The positive rate of NF-κB was 75.3 and 32.9%, respectively; while the positive rate of PTEN was 67.1 and 90.4%, respectively. The positive expression of NF-κB and PTEN was statistically significant. There was a negative correlation between NF-κB and PTEN expression (r=-0.502, p<0.05). The positive and negative expression of NF-κB and PTEN was statistically significant for the five-year survival (p<0.05). At gene and protein level, osteosarcoma tissues had higher expression of NF-κB, and lower expression of PTEN, which was significantly different from the adjacent tissues. In osteosarcoma, NF-κB is highly expressed, but PTEN is expressed at low level, and the two are negatively correlated. This is of great significance for the early diagnosis of osteosarcoma and prognosis.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Gong, T., Su, X., Xia, Q., Wang, J., & Kan, S. (2017). Expression of NF-κB and PTEN in osteosarcoma and its clinical significance. Oncology Letters, 14, 6744-6748. https://doi.org/10.3892/ol.2017.6972
MLA
Gong, T., Su, X., Xia, Q., Wang, J., Kan, S."Expression of NF-κB and PTEN in osteosarcoma and its clinical significance". Oncology Letters 14.6 (2017): 6744-6748.
Chicago
Gong, T., Su, X., Xia, Q., Wang, J., Kan, S."Expression of NF-κB and PTEN in osteosarcoma and its clinical significance". Oncology Letters 14, no. 6 (2017): 6744-6748. https://doi.org/10.3892/ol.2017.6972