Inhibitory effect of the low‑toxic exogenous aryl hydrocarbon receptor modulator 3'3‑diindolylmethane on gastric cancer in mice

  • Authors:
    • Mingli Su
    • Chenchen Qian
    • Yumin Hu
    • Wenhua Lu
    • Rongkang Huang
    • Minhu Chen
    • Jie Chen
  • View Affiliations

  • Published online on: October 16, 2017     https://doi.org/10.3892/ol.2017.7185
  • Pages:8100-8105
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Abstract

3'3‑Diindolylmethane (DIM) has been proved to exhibit anticancer properties in many solid tumors. In our previous study, we demonstrated that DIM inhibited SGC7901 cell proliferation by inducing apoptosis and delaying cell cycle progression. Herein, we further explored the anti‑tumor effect of DIM on SGC‑7901 tumor bearing mice. Tumors were excised, weighed, and tested by western blot and TdT‑UTP nick‑end labeling (TUNEL) assay. Blood samples were collected for biochemical analysis. The expression levels of AhR and cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1) protein were evaluated by western‑blot assay. Our data show that with the increase of DIM dose (0, 5, 10, 20 mg/kg/day), AhR protein gradually decreased as CYP1A1 protein increased. The weight of the tumors found in the treated animals was significantly lower than that of the control group (0.845±0.096 vs. 1.275±0.236 g, 0.768±0.161 vs. 1.275±0.236 g, 0.607±0.106 vs. 1.275±0.236 g, P<0.05). TUNEL test showed that DIM induced increased apoptosis in the treatment groups in a dose‑dependent manner. Blood tests also indicated that DIM showed no toxic effect on animal weight or liver and kidney function. These results indicated that DIM agent could be a safe and potent drug in therapy of gastric cancer.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
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APA
Su, M., Qian, C., Hu, Y., Lu, W., Huang, R., Chen, M., & Chen, J. (2017). Inhibitory effect of the low‑toxic exogenous aryl hydrocarbon receptor modulator 3'3‑diindolylmethane on gastric cancer in mice. Oncology Letters, 14, 8100-8105. https://doi.org/10.3892/ol.2017.7185
MLA
Su, M., Qian, C., Hu, Y., Lu, W., Huang, R., Chen, M., Chen, J."Inhibitory effect of the low‑toxic exogenous aryl hydrocarbon receptor modulator 3'3‑diindolylmethane on gastric cancer in mice". Oncology Letters 14.6 (2017): 8100-8105.
Chicago
Su, M., Qian, C., Hu, Y., Lu, W., Huang, R., Chen, M., Chen, J."Inhibitory effect of the low‑toxic exogenous aryl hydrocarbon receptor modulator 3'3‑diindolylmethane on gastric cancer in mice". Oncology Letters 14, no. 6 (2017): 8100-8105. https://doi.org/10.3892/ol.2017.7185