Open Access

Roles of circulating endothelial progenitor cells and endothelial cells in gastric carcinoma

  • Authors:
    • Bojing Li
    • Zhihong Nie
    • Denghai Zhang
    • Jian Wu
    • Bin Peng
    • Xiaoyan Guo
    • Yihai Shi
    • Xiaoyan Cai
    • Limin Xu
    • Fanfan Cao
  • View Affiliations

  • Published online on: October 26, 2017     https://doi.org/10.3892/ol.2017.7272
  • Pages:324-330
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the role of endothelial progenitor cells (EPCs) and endothelial cells (ECs) in the peripheral blood of patients with gastric cancer (GC), and to investigate vascular endothelial growth factor (VEGF) expression and microvessel density (MVD) in GC tissues. First, 6 ml peripheral blood with added anticoagulant was collected from each of the 42 patients with GC, followed by determination of the number of EPCs and ECs by flow cytometry using the surface markers cluster of differentiation (CD)34brightCD133+CD31+CD45dim and CD34dimCD133‑CD31brightCD45‑, respectively. VEGF expression in patients with GC was detected by the streptomycin avidin‑peroxidase immunohistochemical method, and MVD was calculated using the marker CD34. EPC and EC levels were positively associated with VEGF expression level, as well as with MVD. VEGF expression was positive in 66.67% GC cases, and its level was significantly associated with tumor‑node‑metastasis (TNM) stage, invasion depth and lymph‑node metastasis (P<0.05). VEGF expression level was also positively associated with MVD. MVD in GC was significantly larger than that in normal tissue (P<0.01), and it was significantly associated with TNM stage (P<0.05), invasion depth (P<0.01) and lymph‑node metastasis (P<0.01). EPCs in the peripheral blood have an important role in GC development, and may be a promising indicator of GC diagnosis and prognosis.

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January 2018
Volume 15 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Li, B., Nie, Z., Zhang, D., Wu, J., Peng, B., Guo, X. ... Cao, F. (2018). Roles of circulating endothelial progenitor cells and endothelial cells in gastric carcinoma. Oncology Letters, 15, 324-330. https://doi.org/10.3892/ol.2017.7272
MLA
Li, B., Nie, Z., Zhang, D., Wu, J., Peng, B., Guo, X., Shi, Y., Cai, X., Xu, L., Cao, F."Roles of circulating endothelial progenitor cells and endothelial cells in gastric carcinoma". Oncology Letters 15.1 (2018): 324-330.
Chicago
Li, B., Nie, Z., Zhang, D., Wu, J., Peng, B., Guo, X., Shi, Y., Cai, X., Xu, L., Cao, F."Roles of circulating endothelial progenitor cells and endothelial cells in gastric carcinoma". Oncology Letters 15, no. 1 (2018): 324-330. https://doi.org/10.3892/ol.2017.7272