Long non-coding RNA HOTAIR up‑regulates chemokine (C‑C motif) ligand 2 and promotes proliferation of macrophages and myeloid‑derived suppressor cells in hepatocellular carcinoma cell lines

  • Authors:
    • Yasuyuki Fujisaka
    • Tomoaki Iwata
    • Keiichi Tamai
    • Mao Nakamura
    • Mai Mochizuki
    • Rie Shibuya
    • Kazunori Yamaguchi
    • Tooru Shimosegawa
    • Kennichi Satoh
  • View Affiliations

  • Published online on: November 1, 2017     https://doi.org/10.3892/ol.2017.7322
  • Pages:509-514
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Abstract

Accumulating evidence demonstrated that Hox antisense intergenic RNA (HOTAIR) serves essential roles in the development and metastasis of several types of cancer. In hepatocellular carcinoma (HCC), high expression of HOTAIR is associated with poor prognosis, and HOTAIR regulates cell migration and proliferation. However, the downstream molecular targets of HOTAIR depend on the cancer cell types, and little is known about the precise molecular mechanisms of HOTAIR involved in cancer development. The present study investigated the role of HOTAIR in HCC cell lines. Notably, the overexpression of HOTAIR in HCC cell lines did not affect cell migration and proliferation capability. In the microarray analysis, C‑C motif chemokine ligand (CCL)2 was identified to be differentially expressed in HOTAIR‑overexpressing cells, and it was confirmed that HOTAIR promotes the secretion of CCL2. Furthermore, it was revealed that the proportion of macrophages and myeloid‑derived suppressor cells (MDSCs) were increased when peripheral blood mononuclear cells were co‑cultured with HOTAIR‑overexpressing cells. Collectively, these data suggest that HOTAIR regulates CCL2 expression, which may be involved in the recruitment of macrophages and MDSCs to the tumor microenvironment.

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January 2018
Volume 15 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Fujisaka, Y., Iwata, T., Tamai, K., Nakamura, M., Mochizuki, M., Shibuya, R. ... Satoh, K. (2018). Long non-coding RNA HOTAIR up‑regulates chemokine (C‑C motif) ligand 2 and promotes proliferation of macrophages and myeloid‑derived suppressor cells in hepatocellular carcinoma cell lines. Oncology Letters, 15, 509-514. https://doi.org/10.3892/ol.2017.7322
MLA
Fujisaka, Y., Iwata, T., Tamai, K., Nakamura, M., Mochizuki, M., Shibuya, R., Yamaguchi, K., Shimosegawa, T., Satoh, K."Long non-coding RNA HOTAIR up‑regulates chemokine (C‑C motif) ligand 2 and promotes proliferation of macrophages and myeloid‑derived suppressor cells in hepatocellular carcinoma cell lines". Oncology Letters 15.1 (2018): 509-514.
Chicago
Fujisaka, Y., Iwata, T., Tamai, K., Nakamura, M., Mochizuki, M., Shibuya, R., Yamaguchi, K., Shimosegawa, T., Satoh, K."Long non-coding RNA HOTAIR up‑regulates chemokine (C‑C motif) ligand 2 and promotes proliferation of macrophages and myeloid‑derived suppressor cells in hepatocellular carcinoma cell lines". Oncology Letters 15, no. 1 (2018): 509-514. https://doi.org/10.3892/ol.2017.7322