Anticancer effect of S‑allyl‑L‑cysteine via induction of apoptosis in human bladder cancer cells

  • Authors:
    • Jin‑Nyoung Ho
    • Minyong Kang
    • Sangchul Lee
    • Jong Jin Oh
    • Sung Kyu Hong
    • Sang Eun Lee
    • Seok‑Soo Byun
  • View Affiliations

  • Published online on: October 26, 2017     https://doi.org/10.3892/ol.2017.7280
  • Pages:623-629
Metrics: HTML 0 views | PDF 0 views
0

Abstract

To examine the anticancer effects of S‑allyl‑L‑cysteine (SAC) in human bladder cancer cells and to identify possible molecular mechanisms, bladder cancer cell lines (HTB5, HTB9, JON, UMUC14, T24, and cisplatin resistant‑T24R2) were incubated with SAC, and cell proliferation was measured using the Cell Counting Kit‑8 assay and clonogenic assay. Cell cycle and apoptosis were evaluated by flow cytometry. Expression levels of apoptosis‑ and cell cycle‑associated proteins were analyzed by western blotting. Proliferation and colony formation in bladder cancer cells was significantly inhibited by SAC treatment in a dose‑dependent manner. SAC treatment significantly enhanced apoptosis and promoted a cell cycle arrest in the S phase. SAC also increased the expression of apoptosis‑related genes, including caspases, poly (ADP‑ribose) polymerase and cytochrome c. SAC had an anticancer effect on bladder cancer cells in vitro, at least partially, through the induction of apoptosis and a cell cycle arrest. SAC is a potential therapeutic agent for the treatment of bladder cancer.

Related Articles

Journal Cover

January 2018
Volume 15 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Ho, J., Kang, M., Lee, S., Oh, J., Hong, S., Lee, S., & Byun, S. (2018). Anticancer effect of S‑allyl‑L‑cysteine via induction of apoptosis in human bladder cancer cells. Oncology Letters, 15, 623-629. https://doi.org/10.3892/ol.2017.7280
MLA
Ho, J., Kang, M., Lee, S., Oh, J., Hong, S., Lee, S., Byun, S."Anticancer effect of S‑allyl‑L‑cysteine via induction of apoptosis in human bladder cancer cells". Oncology Letters 15.1 (2018): 623-629.
Chicago
Ho, J., Kang, M., Lee, S., Oh, J., Hong, S., Lee, S., Byun, S."Anticancer effect of S‑allyl‑L‑cysteine via induction of apoptosis in human bladder cancer cells". Oncology Letters 15, no. 1 (2018): 623-629. https://doi.org/10.3892/ol.2017.7280