Down‑expression of poly(ADP‑ribose) polymerase in p53‑regulated pancreatic cancer cells

  • Authors:
    • Zhenyu Hou
    • Yunfeng Cui
    • Huizhi Xing
    • Xiaoyan Mu
  • View Affiliations

  • Published online on: November 29, 2017     https://doi.org/10.3892/ol.2017.7500
  • Pages:1943-1948
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Abstract

The present study investigated whether poly(ADP‑ribose) polymerase (PARP) has an effect on p53‑regulated pancreatic cancer. The results of the present study demonstrated that the expression of PARP affects proliferation and apoptosis of pancreatic cancer cells. Olaparib was used to suppress the expression level of PARP‑1 in PanC‑1 cells. Decreased expression of PARP‑1 suppressed cell proliferation and induced apoptosis of PanC‑1 cells when compared with controls. Furthermore, decreased expression of PARP‑1 resulted in decreased levels of pro‑caspase‑3 expression, increased caspase‑3 activity, suppressed B‑cell lymphoma 2 (Bcl‑2) protein expression and increased p53 protein expression in PanC‑1 cells. Subsequently, ataxia telangiectasia mutated (ATM) activity was inhibited alongside down‑expression of PARP‑1 resulting in significantly decreased cellular viability of PanC‑1 cells, increased p53 protein expression, decreased expression of pro‑caspase‑3, increased caspase‑3 activity and suppressed Bcl‑2 protein expression, when compared with PARP‑1 suppression alone. Overall, the in vitro data confirmed that down‑expression of PARP‑1 suppressed cell proliferation and induced apoptosis of pancreatic cancer via ATM‑deficient p53 signaling pathway.

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February 2018
Volume 15 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Hou, Z., Cui, Y., Xing, H., & Mu, X. (2018). Down‑expression of poly(ADP‑ribose) polymerase in p53‑regulated pancreatic cancer cells. Oncology Letters, 15, 1943-1948. https://doi.org/10.3892/ol.2017.7500
MLA
Hou, Z., Cui, Y., Xing, H., Mu, X."Down‑expression of poly(ADP‑ribose) polymerase in p53‑regulated pancreatic cancer cells". Oncology Letters 15.2 (2018): 1943-1948.
Chicago
Hou, Z., Cui, Y., Xing, H., Mu, X."Down‑expression of poly(ADP‑ribose) polymerase in p53‑regulated pancreatic cancer cells". Oncology Letters 15, no. 2 (2018): 1943-1948. https://doi.org/10.3892/ol.2017.7500